SNPs analysis of CR2 and PIGR. (A) The CR2 IVS2-848C→T was distinguishable by TaqI restriction. Digestion of the 1241 bp amplicon yielded two DNA fragments, 750 and 491 bp. Lane 1, 3 and 4 were homozygous -/- and lane 2 was heterozygous +/-. (B-D) PIGR polymorphisms was investigated by PCR-RFLP and ARMS. (B) RFLP analysis of 1392 bp PIGR IVS3-156G→T PCR products with PvuII digestion yielded 1163 and 229 bp DNA fragments. Lane 1,2,4,7 and 8 were heterozygous +/-, samples 3,9 and 12 were homozygous +/+, and samples 5,6,10 and 11 were homozygous -/-. (C) RFLP of PIGR 1739C of the 220 bp PCR product was analyzed by HgaI digestion and yielded two fragments of 180 and 40 bp, respectively, whereas 1739T remained as 220 bp DNA product. Heterozygous CT yielded 220,180 and 40 bp fragments. Negative control in lane1; homozygous CC in lanes 2,5,7 and 8; heterozygous CT in lanes 3 and 6, and homozygous TT in lane 4. (D) PIGR 1093G→A was detected by ARMS. Negative control in lanes 1 and 2 for primer sets A and B. Samples S1, 2, 3, were homozygous 1093G. Samples S4, 5, 6 were homozygous 1093A, and S7 was heterozygous.