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Figure 6 | BMC Genetics

Figure 6

From: Parasexual genetics of Dictyostelium gene disruptions: identification of a ras pathway using diploids

Figure 6

Production of a ras S/gef B double mutant by parasexual recombination. (A) Schematic diagram showing the recombination process (B) PCR screen for ras S disruption. Wild-type alleles give a 450 bp product, disrupted alleles give a product of 3000 bp.(C) PCR screen for gef B disruption. Wild-type alleles give a 480 bp product, disrupted alleles give a product of 1550 bp.

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