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Table 5 Fluctuation tests and analysis of mutations under selective conditions

From: Occurrence of leu+ revertants under starvation cultures in Escherichia coli is growth-dependent

Exp.>A Media /Mutation Cultures with no mutants/total cultures Number of mutants MeanB VarianceC Distribu-tionD
1–1 LB leu - leu + 3/10 1,1,1,2,2,2,2 1.10 0.767 P
1–2 CM leu - leu + 0/10 16,16,18,19,21,21,24, 24,27,28 22.60 18.267 P
1–3 MM+P leu - leu + 0/10 519,552,526,612,572,634,632,619, 601,597 586.4 1774.5 P
1–4 PB leu - leu + 0/10 1,1,2,2,2,,3,3,4,5,8 3.10 4.545 P
2–1 LB leu - leu + 34/45 3,16,27,37,85,116,146,326,680,372,1284 90.64 63989 LD
2–2 CM leu - leu + 16/45 2,5,7,7,9,9,10,12,13,14,14,16,16,16,18,18,19,19,30,35,42,44, 48,50,62,75,104,126,231 23.80 1770.3 LD
2–3 MM+P leu - leu + 22/45 1,2,2,3,3,4,6,6,8,8,9,10,11,13, 15,19,23,24,25,32,55,67,103 9.98 402.20 LD
3–1 MM+P leu - leu + 14/45 1,1,1,3,3,4,4,13,21,66 4.875 193.42 LD
3–2 MM+P leu - leu + 4/24 1,1,1,2,2,3,3,3,4,4,5,5,7,7,9,12,12,19,26,50 7.333 123.19 LD
3–3 MM+P leu - leu + 11/24 1,1,1,2.3.7.9.12.14,21,29,33,38 7.125 134.03 LD
4–1 MM+L pro - pro + 14/24 1,1,1,1,3,4,9,13,15,41 3.708 80.65 LD
4–2 MM+L Pro - pro + 14/24 1,1,1,2,2,2,4,5,7,9,18,34 3.583 59.04 LD
4–3 MM+L pro - pro + 14/24 1,1,1,1,2,2,3,3,5,11,11,12, 24,42 4.958 95.26 LD
5–1 CM-lac lac - lac + 19/24 1,1, 4,23,38 2.792 78.43 LD
5–2 CM-lac lac - lac + 14/24 1,1,1,1,2,2,2,6,30,44 3.750 110.89 LD
5–3 CM-lac lac - lac + 11/14 1,1,1,2,2,2,3,3,5,6,9,14,72 5.042 214.99 LD
  1. A: Experiments in group 1 (1–1 to 1–4) , aliquots from cultures in same flasks; experiments in other groups, each cultures were independent in a little tube or in a well of 48 wells microtittle. B: Mean of mutants., here, n = number of cultures, xi = number of mutants in a culture. C: Variance of simples.D: distribution patterns were judged by the Value of X2. n = 10□ a0.95 = 3.94; n = 24, a0.95= = 13.848; n = 45 a0.95 = 30.612. If the value of X2 > a0.95, the distribution type was non-Poisson pattern; If the value of X2 < a0.95, the distribution type was Poisson pattern.