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Table 3 Leucine leaked from scavenger bacteria supporting the cells' growth and mutation

From: Occurrence of leu+ revertants under starvation cultures in Escherichia coli is growth-dependent

Number of cells per culture at starting time Number of leu + revertants in different media
Time of scavenger bacteria culturing (day)
0 6 hours 24 hours 48 hours 120 hours 168 hours
2.89 × 105 0 0 0 0 0 0
2.89 × 106 0 0 0 0 0 0
2.89 × 107 0 0 0 1 2 1
2.89 × 108 0 1 1 5 4 5
2.89 × 109 0 2 3 17 21 17
Total revertants 0 3 4 23 27 25
CFUA/culture 2.31 × 105 1.36 × 106 3.54 × 106 8.53 × 106 3.73 × 106 3.02 × 106
CFUB/culture 7.64 × 107 1.69 × 108 3.23 × 108 3.69 × 108 4.46 × 108 4.10 × 108
Leucine (ng/ml) ------ 578.7 842.3 971.9 963.2 962.1
  1. A: The number of viable cells of 31343 which were cultured in the medium at 37°C for 24 hours. The initial number of 31343 cells were 2.89 × 105 cells/ml. The medium was MM+P in which the scavenger bacteria cells (in the dialysis tubings) of 35577 were pre-cultured for different days at the concentration of 7.97 × 108 cells/ml. B Same as A except that the initial number of 31343 cells was 2.89 × 108 cells/ml.