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Table 2 Leu+ reversions under selection were cell density-dependent

From: Occurrence of leu+ revertants under starvation cultures in Escherichia coli is growth-dependent

  Description Number of micro-drops or plates with Ni papillae
  Day 2 Day 4 Day 7 Day 11 Day 15 Day 18
A 8.27 × 107 cells/micro-drop N0 = 589 N0 = 501 N0 = 475 N0 = 462 N0 = 344 N0 = 264
  Total micro-drops = 620 N1 = 29 N1 = 108 N1 = 125 N1 = 116 N1 = 199 N1 = 246
  Total cells = 5.13 × 1010 N2 = 2 N2 = 11 N2 = 13 N2 = 20 N2 = 49 N2 = 63
     N3 = 7 N3 = 9 N3 = 23 N3 = 31
      N4 = 3 N4 = 5 N4 = 13
        N5 = 3
  Papillae/micro-drop 0.053 0.210 0.277 0.315 0.619 0.858
B 8.27 × 107 cells/plate N1 = 6 N1 = 8 N2 = 1 N1 = 8 N1 = 8 N1 = 7 N1 = 7
  Total plates = 50    N2 = 2 N2 = 2 N2 = 2 N2 = 2
  Total cells = 4.20 × 109      N3 = 1 N3 = 1
  Papillae/plate 0.12 0.20 0.24 0.24 0.28 0.28
C 8.27 × 106 cells/micro-drop N0 = 1600 N0 = 1600 N0 = 1600 N0 = 1599 N0 = 1598 N0 = 1598
  Total micro-drops = 1600     N1 = 1 N1 = 2 N1 = 2
  Total cells = 1.32 × 1010       
  Papillae/micro-drop 0 0 0 0.001 0.001 0.001
D 8.27 × 107 cells/micro-drop N0 = 160 N0 = 154 N1 = 6 N0 = 152 N0 = 145 N0 = 137 N0 = 127
  Total micro-drops = 160    N1 = 8 N1 = 15 N1 = 23 N1 = 33
  Total cells = 1.32 × 1010       
  Papillae/micro-drop 0 0.038 0.05 0.094 0.144 0.206
E 2.96 × 108 cells/micro-drop N0 = 45 N0 = 41 N0 = 33 N0 = 21 N0 = 11 N0 = 8
  Total drops = 125 N1 = 38 N1 = 39 N1 = 42 N1 = 40 N1 = 45 N1 = 42
   N2 = 24 N2 = 24 N2 = 27 N2 = 37 N2 = 40 N2 = 38
   N3 = 11 N3 = 12 N3 = 13 N3 = 12 N3 = 13 N3 = 19
   N4 = 6 N4 = 7 N4 = 8 N4 = 12 N4 = 11 N4 = 12
   N5 = 1 N5 = 2 N5 = 2 N5 = 3 N5 = 5 N5 = 6
  Papillae/micro-drop 1.18 1.29 1.42 1.70 1.90 2.02
F 2.96 × 108 cells/micro-drop N0 = 125 N0 = 125 N0 = 125 N0 = 125 ------ ------
  Total drops = 125       
  Papillae/micro-drop 0 0 0 0 ----- ------
  1. A,C,E: Cells were deposited as micro-drops on MM+P plates. B: Cells were spread on MM+P plates. D: cells were pre-cultured in 0.85% NaCl solution for 14 hours at 37°C before deposited as micro-drop on MM+P plates. F: Cells were deposited as micro-drops on MM+P plates with 100 μg/ml Ampcillin. Ampicillin was added once a day with 1 ml (1000 μg/ml) around the every micro-drops till day 7.