Human CCS gene: genomic organization and exclusion as a candidate for amyotrophic lateral sclerosis (ALS)

BMC Genomic Data

Table 2 Primers and PCR conditions used for analysis of CCS gene

Exon	Forward	Reverse	Product Size (bp)	Annealing Temp (°C)^*
1	CGCCGCGCTGGTTGGTG	CGGCGAGGCTCCACACGAAG	138	57
2	CGATCATCGGTTGGT	TAATAGATTCGCCTGGTC	173	51
3	CAGCCTCTGTTGCCCTCT	ACCTTCCTCAAAGCCACTCC	285	57
4	CAAGCCAGCCCAAGTGTCTA	TGAGGGCCCAGTGTGAAA	271	57
5	CCTGTGAGCTGCTTTCCT	TCTGAGACTTTGGGTCCTCT	159	57
6	ATAGCAGCTTGGCACCAC	GATGATGGACCCTGTTAGGA	184	57
7	TGGGCACCCTTCCTAACAG	ACCATCCGCAGAGCACAG	201	57
8	CACCCCTGACCACACATT	ATAAGGGGCATGGAGACAA	379	57

^*PCR conditions: initial denaturation at 94°C for 5 min; 40 cycles of denaturation at 94°C (30 s), annealing (30 s), extention at 72°C (40 s); final extension at 72°C for 7 min.

ISSN: 2730-6844