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Figure 3 | BMC Genetics

Figure 3

From: Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae

Figure 3

tab3-1 enables TEM1-independent degradation ofClb2 and accumulation of Sic1. tem1Δ::GAL1-UPL-TEM1 tab3-1 (WY97) and tem1Δ::GAL1-UPL-TEM1 (WY46) cells grown in galactose medium (TEM1 expressed) at 25°C were arrested in G1 with α factor, and released into glucose medium (TEM1 repressed) at time = 0. (A) At indicated time points after release, budding index was monitored. (B) The experiment in (A) was repeated, except that at either 2 h (TAB3) or 3 h (tab3-1) following release from α factor arrest, α factor was added back to prevent cells from proceeding through a second cell cycle. At indicated time points, samples were withdrawn to measure Sic1 and Clb2 levels by SDS-PAGE and immunoblotting.

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