From: Screening of the arrestin gene in dogs afflicted with generalized progressive retinal atrophy
Primer | Location | Forward primer, reverse primer (5'–3')a | PCR conditions [T-°C/MgCl2-mM] | PCR amplicon length (bp) | Restriction enzymes for SSCP analyses |
---|---|---|---|---|---|
UTR1F | exon 1 | GGGCAACCCTGTCCAGGT | 54/1.0 | 699 | Nla III/Rsa I |
UTRI-1aR | intron 1 | TCTATCATGACGGGACGCCT | |||
UTRI-1aF | intron 1 | GAAAATGATATTTGCAAAGCAG | 50/1.0b | 283 | Tru 1I |
UTRI-1aR | intron 1 | TCTATCATGACGGGACGCCT | |||
1-IF | intron 1 | CTAATGGGCACACAGCATCTC | 53/1.0 | 249 | Pvu III; Nla IIIc |
2-I2R | intron 2 | TTCTGTTAAGCCACTCACTTC | |||
3-IF | intron 2 | TGTTTTTATCTAACACTGACTACTTC | 48/1.0b | 224 | Nla III |
3-IR | intron 3 | AAATAACAAAGTAGCAGCTGTC | |||
4-IF | intron 3 | AACTGCAGATAAATATATGAAG | 52/1.0 | 189 | Alu I |
4-IR | intron 4 | AAAGTTCTTTCCTAGCACTAAG | |||
5-IF | intron 4 | GGTTACCCCATGTTCACTTG | 56/1.0 | 343 | Mnl I |
5-IR | intron 5 | GCTCCTGGTCACACTGCAAG | |||
6-IF | intron 5 | CAAGTTTTACACACTGAGTGC | 55/1.0 | 213 | Alu I |
6-IR | intron 6 | ATTTTCCCCAGAGAAAAGGCTA | |||
7-IF | intron 6 | CGGGAAGGGAGGTGCTGA | 58/1.0 | 233 | Rsa Ic |
7-IR | intron 7 | TCGCAGGCCACAGAGGAGAAG | |||
8-IF | intron 7 | ATCACAGCGTGAGTACGGGGAG | 55/1.0 | 288 | RsaI; Pst Ic |
8-IR | intron 8 | CAGCACCCACAGCAGATTG | |||
9-IF | intron 8 | CCTAGAAAGCCATGAGATTAA | 55/1.5 | 214 | Tru I1; Sty Ic |
9-IR | intron 9 | GACCAGACTGAGAAATTCTAG | |||
10-IF | intron 9 | GGCACCATGCACATGCGTG | 58/1.0 | 235 | Alu I |
10-IR | intron 10 | AAGCCAAGCATCCTACTTCC | |||
11-IF | intron 10 | GGACTGATGGTGGCTTTATG | 58/1.0 | 266 | BsuR I |
11-IR | intron 11 | AGCAGACCAGCACCTCCTC | |||
12-IF | intron 11 | TGAGGGATGTGTTCATCTAG | 55/1.0 | 183 | - |
12-IR | intron 12 | GCCCATGGTGTTGGCTCTTG | |||
13-IF | intron 12 | CATGCTTGGGACATGTCCAC | 64/1.0 | 209 | Mva I |
13-IR | intron 13 | TTCATAACACCTCTGAGCTAC | |||
14-IF | intron 13 | CTCTGCAGCCACAGCCCTTC | 50/2.0 | 229 | Ava II |
14-IR | intron 14 | GTGCTCAGGGACCACTAG | |||
15-IF | intron 14 | CCTGCTCACGATTCTCTTTC | 58/1.0 | 244 | Hph I |
15-IR | intron 15 | AAGCCGAAGACTGGGAGC | |||
16-IF | intron 15 | GATCGGTCCCTTGTTGCA | 52/2.0b | 347 | Alu I |
16-ER | Exon 16 | CACAGCTGAACAGACAAACTT |