Table 2 Primers for the characterization of the SAG gene
From: Screening of the arrestin gene in dogs afflicted with generalized progressive retinal atrophy
Exon | Forward primer, reverse primer (5'–3') | Exon (#) and size (bp) | Intron size (bp) | splice donor site (gt ), splice acceptor site (ag ) |
|---|---|---|---|---|
1–2 | GGGCAACCCTGTCCAGG | (1) 156 | 836 | |
ACACCCTGGGGTCTGTGTC | CACAAGgt acatg | |||
2–3 | AGTGAAAGAAGCTACCAGGGA | (2) 132 | ~ 5500 | ttcccag CTTGCT |
ACCCACAGGCTCTACTTGT | AAGTCGgt aagtgg | |||
3–4 | AGGTGACCATCTACTTGGG | (3) 61 | ~ 2600 | cctatag GTGACC |
AAAGTTCTTTCCTAGCACTAAGa | CTGTGGgt aagttg | |||
4–5 | AGATGGTATCGTGTTGGTGG | (4) 45 | ~ 1800 | ggtttag ATGGTA |
ACCGTGAGCAGGAAGGG | AGAAAGgt aagaca | |||
5–6 | AGTGTATGTCTCTCTGACCTG | (5) 194 | ~ 2000 | cctccag TGTATGT |
ACCTTCCCCATGTCTTGCG | CTCACGgt gggtg | |||
6–7 | CAAGTTTTACACACTGAGTGCa | (6) 60 | ~ 1300 | cccacag TTCCCTG |
TCGCAGGCCACAGAGGAGAAGa | GGGAAGgt tagtg | |||
7–8 | GTGCTGTGGGGTCGACTTT | (7) 77 | ~ 1500 | actgcag TGCTGTG |
CAGCACCCACAGCAGATTGa | CAAGAAgt aagagt | |||
8–9 | AGGAGGTCCGTGCGTTTTAC | (8) 136 | 905 | tctgcag GAGCTCC |
CTAATGCCTTTAATCTTCTTC | AAAGAGgt gagcca | |||
9–10 | CCTAGAAAGCCATGAGATTAAAa | (9) 85 | ~ 1500 | ttttcag ATCTATT |
AAGCCAAGCATCCTACTTCC | CATTAGgt aggaac | |||
10–11 | TGGAACAAGTGGCCAACGTTa | (10) 73 | ~ 2500 | tctgcag TGGAACA |
ACATGGTGCTGCAAGG | AACACAgt gagtag | |||
11–12 | GGACTGATGGTGGCTTTATGa | (11) 138 | ~ 3200 | cctacag AGAAAAA |
ACCCTGACACCGTGAGCTTC | CACCATgt gagtcc | |||
12–13 | TGAGGGATGTGTTCATCTAGa | (12) 78 | 1390 | tgagcag AATAAAG |
TTCATAACACCTCTGAGCTACa | GTCAGGgt gagtgt | |||
13–14 | CTGGAGGTGAGCTCTCCCA | (13) 24 | ~ 1400 | ttcctag CTTTCTG |
GTGCTCAGGGACCACTAG | CTCCAGgt aagcct | |||
14–15 | AGTGAAGTGGCAACTGAGGT | (14) 56 | ~ 3000 | tttccag TGAAGTG |
AAGCCGAAGACTGGGAGCa | ACCCAGgt cagtcg | |||
15–16 | CCTGCTCACGATTCTCTTTC | (15) 16 | 426 | cttacag CTACGGC |
GTCCAGGTCGACCGACCT | GGAAAGgt gagccc | |||
(16) 237 | tcttttag TTTTCA |