Figure 3

Expression of Tnfrh1 in adult and fetal mouse tissues. Tnfrh1 mRNA is detected as two alternative transcripts, both of which are present in most tissues. Both transcripts are most abundant in the placenta. The Tnfrh1 partial cDNA used to probe these blots has no detectable sequence identity with Tnfrh2 over the 5'-most 65 nt, but has 91% identity with the Tnfrh2 over the remaining 450 nt. Stripping and rehybridization at high stringency, with a smaller 5' probe with 72% overall sequence identity over 297 nt gave an identical pattern of bands. Ethidium bromide (EtBr) staining of ribosomal RNA is a loading control. The lanes contain 20 micrograms of total RNA; blots were exposed overnight.