Figure 4

A) The expression of UNC93A in ovarian cancer cell lines by RT-PCR. Top panel: Ethidium-bromide stained agarose gel of amplification products from the RT-PCR using primers located in exon 1 and exon 8 of the cDNA of UNC93A to amplify the entire coding sequence. The template for the RT-PCR were the total RNAs from indicated ovarian cancer cell lines (2 μg each). Two products of 1.4 kb and 1.3 kb were observed in 41M and OAW28. Bottom panel: GAPDH was amplified by RT-PCR using the same panel of RNAs as those used in the RT-PCR of the top panel. B) Amplification of exon 3–5 by RT-PCR . Top panel: Ethidium-bromide stained agarose gel of amplification products from the RT-PCR using primers located in exon 3 and exon 5 of the cDNA of UNC93A. The template of the RT-PCRs were the total RNAs from normal ovary tissue, normal placenta tissue and 8 ovarian cancer cell lines (2 μg each). A major RT-PCR amplicon about 550 bp was observed in all the samples except in the negative control. Another amplicon about 400 bp was observed in cell lines 41M, OAW28. Bottom panel: GAPDH was amplified by RT-PCR using the same panel of RNAs as those used in the RT-PCR of the top panel. C) Northern blot analysis of the UNC93A expression in adult tissues. A Northern blot of poly (A)⁺ mRNA (2 μg each lane) from 8 adult tissues (Clontech) was hybridised to full length cDNA of UNC93A. The membrane was exposed to Fuji film at -70°C for 5 days. Two major transcripts of 1.1 and 2.2 kb were observed in spleen, prostate, testis, ovary, small intestine, and colon.