Figure 4
From: NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae
Mutations in SNF1 and SNF/SWI can be suppressed by both nrg1Δ and miglΔ. A single colony of each strain was inoculated into YPD liquid and grown over-night to saturation and adjusted in water to 1 × 108 cells/ml. The cultures were then diluted 1:2 in sterile water and spotted on YPD, YP galactose and YP sucrose plates, with uracil added to each plate to 80 μM. The first spot of each row represents a cell count of 5 × 107 cells/ml, which is diluted 1:4 for the second spot and 1:2 for each spot thereafter. YPD and YP sucrose plates were photographed after incubation at 30°C for 2 days, and YP galactose plates were photographed after 5 days.