Table 1 Locus abbreviations, enzymes stained for, enzyme commission numbers (E. C. no.) as well as the tissues and buffers giving optimal results (buffer descriptions are given in Materials and Methods).
Protein | Locus | E.C. No. | Buffer | Tissue |
|---|---|---|---|---|
1. Aspartate aminotransferase | AAT-1, -2 | 2.6.1.1 | P | Bl |
2. Alcohol dehydrogenase | ADH | 1.1.1.1 | TC | L |
3. Creatine kinase | CK-1 to -3 | 2.7.3.2 | TC | M |
4. Esterases | EST-1 to -3 | 3.1.1.- | RW, MF | M, L |
5. General proteins | PT-1 to -5 | Â | PAGE | M |
6. Glucose-6-phosphate isomerase | GPI-1, -2 | 3.5.1.9 | RW, P | L |
7. Glyceraldehyde-3-phosphate dehydrogenase | GAP | 1.2.1.12 | TC | M, L |
8. Glycerol-3-phosphate dehydrogenase | GPD-1 to -4 | 1.1.1.8 | RW | M, L |
9. Haemoglobin | Hb-1, -2 | Â | PAGE | Bl |
10. Isocitrate dehydrogenase | IDH-1 to -3 | 1.1.1.42 | TC | M, L |
11. L-Lactate dehydrogenase | LDH-1, -2 | 1.1.1.27 | RW | Bl, M |
12. Malate dehydrogenase | MDH-1, -2 | 1.1.1.37 | TC | M, L |
13. 6-Phosphogluconate dehydrogenase | PGD-1 to -3 | 1.1.1.44 | HC | M, L |
14. Phosphoglucomutase | PGM-1 to -3 | 5.4.2.2 | P, RW | M |
14b Phosphoglucomutase | PGM-4 | 5.4.2.2 | RW | L |
15. Superoxide dismutase | SOD-1, -2 | 1.15.1.1 | P, RW | M, L |