No effect of 1X anesthetic dose within 12 minutes of administration. A Mean IOP ± SD is shown for C57BL/6J mice at 5 and 25 minutes after administration of various doses of anesthetic. The 1X dose consisted of 99 mg/kg ketamine and 9 mg/kg xylazine. All doses decreased IOP by 25 minutes. At all doses the IOP at 5 minutes was the same suggesting that the effect of anesthesia had not yet occurred. Approximately thirty 3 to 4 month old mice were analyzed at each dose and time B-D. Scatter-plots demonstrating no change in IOP over the 12 minutes following anesthetic administration in three relatively unrelated mouse strains. All strains consisted of males and females that ranged from 3 to 6 months of age. The sexes and ages were equally represented at each time point. The scatter-plots include 195 C57BL/6J, 161 129P3/J and 145 DBA/2J mice.