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Table 1 Population surveys of D negative blood donors documented D negative and screened by RHD PCR-SSP

From: RHD positive haplotypes in D negative Europeans

 

Samples (n)

Documented phenotype

screened

PCR-SSP positive

D positive*

Testing as single samples †

   Ccddee

433

34

0

   ccddEe

271

5

2

   CCddee

24

4

0

   CcddEe

19

4

2

   ccddEE

6

1

0

   CcddEE

1

0

0

   ccddee

314

0

0

Testing as pools of 20 samples ‡

   ccddee

7,374

2

1

Total

 

50

5

  1. *Samples uncovered on further analysis as weak D, partial D or D+/- chimera. † Positive for at least one of four RHD specific polymorphism tested (promoter, intron 4, exon 7 and 3' UTR). ‡Positive for at least one of three RHD specific polymorphism tested (promoter, intron 4, and 3' UTR).