Figure 3

Demonstration of the RHCE(L245V) and RHD(N152T) substitutions characteristic of Cde^s. PCR-SSP were performed to detect single nucleotide polymorphism characteristic for Cde^s [16] and indicative of RHCE(L245V) (lanes 1 to 4, 110 bp specific product) and RHD(N152T) (lanes 5 to 8, 120 bp specific product). Both polymorphism were present in the Cde^s sample as expected (lanes 1 and 5). The RHD-CE(4-7)-D₂ sample was compatible with Cde^s according to the RHD exon specific PCR (Fig. 1) but lacked both polymorphism (lanes 2 and 6). Negative controls were standard RHD (lanes 3 and 7), positive controls weak D type 4 (lane 4) and D^III type IV (lane 8), respectively.