Figure 4From: Genetic analysis, structural modeling, and direct coupling analysis suggest a mechanism for phosphate signaling in Escherichia coliSignaling activity of various PhoU mutants. Triplicate cultures were grown in LB and assayed for Bacterial Alkaline Phosphatase activity. BW25113 is a wildtype control. Other strains were STACΔphoU with p116U2 (wildtype phoU), pKG116 (empty parent plasmid), and various p116U2 plasmids with specific mutations in phoU (A147E, A147K, R148A, R148E, and D150A). Error bars represent standard deviations. Values significantly greater than p116U2 are labeled with * = p-value of < 0.001 compared to p116U2 with a two-tailed T-test.Back to article page