Figure 2

Marker gene expression in transgenic Lucilia cuprina. Adult flies or embryos were observed with either white light (A,E), a long pass GFP filter set (B,D) or a DsRed filter set (C,F). (A-C) Young adults (less than 2h after eclosion) from the wild-type stock (left), a transgenic line that carries the ZsGreen marker (center) and a line that has the DsRed-Express 2 (DsRedex2) marker (right) are shown. (D) A mixture of non-transgenic and transgenic (ZsGreen marker) late stage embryos. The transgenic embryos show strong green fluorescence and are easily separated from the non-transgenic embryos. (E,F) Embryos from the wild-type stock (left) and from a transgenic line that carries the DsRedex2 marker (right). Transgenic individuals show bright green or red fluorescence in all cells from the mid-embryo to young adult stage. In older adults that have darker cuticles, fluorescence is much more difficult to detect (not shown). Consequently, we routinely screen for transgenic individuals at the embryo and larval stages. (G,H) Schematic representations of the pB[Lchsp83-ZsGreen] and pB[Lchsp83-DsRedex2] transformation vectors The vectors contains the 5' flanking DNA, first exon and first intron from the Lchsp83 gene.