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Figure 5 | BMC Genetics

Figure 5

From: SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans

Figure 5

Localization of a spe-8::GFP translational reporter in developing sperm. GFP was fused to the C-terminus of the spe-8 coding sequence and integrated into a Mos1 site on Chromosome II following the MosSCI technique. In the N2 column, cells from the unlabeled N2 strain are included as a negative control to illustrate the non-specific green autofluorescence found in C. elegans developing sperm. The spe-8::GFP column shows the green fluorescence in cells with the translational reporter. Note the arrows indicating membrane labeling not seen in the N2 controls. The remaining columns are from worms homozygous for the reporter construct insertion and a mutation in one of the spe-8 group genes. Membrane GFP localization was absent in spe-12(hc76), spe-19(ok3428), and spe-27(it132) mutant backgrounds, but some localization was present in a spe-29(it127) mutant background. Red fluorescence indicates DNA labeling by Hoechst 33342.

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BMC Genomic Data

ISSN: 2730-6844

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