Table 2 Details of the primers used
Primer name | Gene | Protein | ANN temp | Exon no. | Start (bp) | End (bp) | Forward primer | Reverse primer |
|---|---|---|---|---|---|---|---|---|
BDTG 1 | Xa1 | P Loop | 59.8 | 1 | 3113 | 3621 | 5 ́-ATTAATCCACGACGACCAGG – 3 ́ | 5 ́-GTAGCACAAGCACCTCCTCC – 3 ́ |
BDTG 2 | Kinase 2 & 3 | 60 | 2 | 3602 | 4031 | 5 ́-GAGGAGGTGCTTGTGCTACAG – 3 ́ | 5 ́-GGCACTGGCATTACCTTGAT – 3 ́ | |
BDTG 3 | TRANS MEM | 59.5 | 3 | 4681 | 5200 | 5 ́-GGTGAGGGTGCATCAAATG – 3 ́ | 5 ́-TTATTCCTTCGTGGCTCTGG – 3 ́ | |
BDTG 4 | 59.8 | 3 | 5167 | 5698 | 5 ́-TTGGATCATGTCTCCAACCA – 3 ́ | 5 ́-ACTTCAGCGCTTGCATGAT – 3 ́ | ||
BDTG 5 | 59.8 | 3 | 5710 | 6587 | 5 ́-CATCTATCCAACCCCTTACAGC – 3 ́ | 5 ́-CAAGCTTGTTCATGGATTTCAA – 3 ́ | ||
BDTG 6 | 60.2 | 3 | 6621 | 8399 | 5 ́-TAGAACTCAGGAGGAGGCATGT – 3 ́ | 5 ́-TGATTGCGGAAGGATACACA – 3 ́ | ||
BDTG 7 | 60.2 | 3 | 8370 | 8940 | 5 ́-AGATGGAATGTGTATCCTTCCG – 3 ́ | 5 ́-GGAAGGATACACCTTCCATTTTC – 3 ́ | ||
BDTG 8 | LRR | 59.5 | 4 | 25981 | 26700 | 5 ́-GATGGCTCCTACCGCTATCA – 3 ́ | 5 ́-GATGTGCAAGAATGGAGCTG – 3 ́ | |
BDTG 9 | 60.9 | 4 | 26662 | 27231 | 5 ́-CTCAAATTTAGTGTCTCTGCAGCTC – 3 ́ | 5 ́-TCCGCGATAGTTAAGCTCTAGG – 3 ́ | ||
BDTG 10 | 60 | 4 | 27182 | 27917 | 5 ́-TCTGCAAGCACCTCACCTC – 3 ́ | 5 ́-ATGCATTGGAGCGGATTG – 3 ́ | ||
BDTG 11 | Xa5 | TF II A | 59.9 | 1 | 406048 | 406306 | 5 ́-TTCGAGCTCTACCGGAGGT – 3 ́ | 5 ́-AGAAACCTTGCTCTTGACTTGG – 3 ́ |
BDTG 12 | 60.2 | 2 | 411394 | 411535 | 5 ́-TGTTCTTTTCTCAGGGCCAC – 3 ́ | 5 ́-AGTTTGGAATCACAGGCCAC – 3 ́ | ||
BDTG 13 | Xa26 | RECP Kinase | 59.5 | 1 | 1500 | 2094 | 5 ́-GATGCATACTCTTGCTGCCA – 3 ́ | 5 ́-CAAGACTGTGCAACCCCTG – 3 ́ |
BDTG 14 | 60.1 | 1 | 2043 | 2695 | 5 ́-ACCAGCTATACGGTCCAATCC – 3 ́ | 5 ́-GCAAGATGCAACCATGAAAGT – 3 ́ | ||
BDTG 15 | 59.6 | 1 | 2716 | 3332 | 5 ́-CTATTCCTGCTTCTCTTGGCA – 3 ́ | 5 ́-AGCCTGACGATTTTATCAAGATG – 3 ́ | ||
BDTG 16 | 59.6 | 1 | 3320 | 3956 | 5 ́-CATCTTGATAAAATCGTCAGGCT – 3 ́ | 5 ́-GGTTGCACGAAGAAGCTCAT – 3 ́ | ||
BDTG 17 | 59.8 | 1 | 3968 | 4492 | 5 ́-CGATGATAGCATGTTGGGC – 3 ́ | 5 ́-AAAAACTATTAAGTACCTGGTGCCAT– 3 ́ | ||
BDTG 18 | 59.9 | 1 | 4574 | 5141 | 5 ́-TGAGCAGAGTATGGGACTCTAGG – 3 ́ | 5 ́-ACACCAACTATAAATTGTTGCAGAAC – 3 ́ | ||
BDTG 19 | Xa27 | 59.9 | 1 | 1518 | 1909 | 5 ́-GAAGCCACACACACTGAGACA – 3 ́ | 5 ́-CGGAGGAGAACTAGAGAGACCA – 3 ́ | |
BDTG 20 | Xa21 | Signal | 59.7 | 1 | 8 | 208 | 5 ́-CACTCCCATTATTGCTCTTCG – 3 ́ | 5 ́-ACACAACACCCACCCATGT – 3 ́ |
BDTG 21 | LRR | 61.8 | 2 | 260 | 760 | 5 ́-GCTCCTCCAACCTGTCCG – 3 ́ | 5 ́-TAAACGCTCTTAGAGACGAAAGGT – 3 ́ | |
BDTG 22 | 59.7 | 2 | 723 | 1314 | 5 ́-CAATTCTATCTGGAACCTTTCGTC – 3 ́ | 5 ́-ACCGCTCAAGTTGTTTTCGT – 3 ́ | ||
BDTG 23 | 60 | 2 | 1279 | 1880 | 5 ́-GGCATTCTACTCGCCTACGA – 3 ́ | 5 ́-GCATTGCCTTGGATTGAGAT – 3 ́ | ||
BDTG 24 | Charged | 59.8 | 3 | 1913 | 2620 | 5 ́-TGCCTCGATGTTGTCCATTA – 3 ́ | 5 ́-TCAATGAGGTCCCATCAACA – 3 ́ | |
BDTG 25 | Kinase | 60.1 | 4 & 5 | 2651 | 3919 | 5 ́-AGGGACAATTGGCTATGCAG – 3 ́ | 5 ́-AGAATTCAAGGCTCCCACCT – 3 ́ | |
BDTG 26 | Xa21(A1) | LRR | 59.8 | 1 | 4802 | 5082 | 5 ́-TGTTGTTCTCTGCGCTGC – 3 ́ | 5 ́-CGTCCTGAGGAAGGATAGGTT – 3 ́ |
BDTG 27 | 59.6 | 1 | 5051 | 5459 | 5 ́-CATCGCTGGGCAACCTAT – 3 ́ | 5 ́-TTGGACACGACTTCAAATATGG – 3 ́ | ||
BDTG 28 | 59.6 | 1 | 5406 | 5803 | 5 ́-CCCAGATCCTATTTGGAACATC – 3 ́ | 5 ́-TGGAAACAGAATCAGGGAGG – 3 ́ | ||
BDTG 29 | 59.9 | 1 | 5763 | 6173 | 5 ́-AGGTTGCAAATTTGGTGGAG – 3 ́ | 5 ́-GGAATGCTAAATATTTCAATGGGA – 3 ́ | ||
BDTG 30 | 60.2 | 1 | 6140 | 6531 | 5 ́-TAGGGCAAATTCCCATTGAA – 3 ́ | 5 ́-AAAACACCATTGGTTGGCA – 3 ́ | ||
BDTG 31 | 59.9 | 1 | 6484 | 6889 | 5 ́-CTTTCGTTCAACAGCTTCCAC – 3 ́ | 5 ́-CACCATCTTGACTATCAAATTCTCC – 3 ́ | ||
BDTG 32 | 59.9 | 1 | 6859 | 7422 | 5 ́-CTTTCGTTCAACAGCTTCCAC – 3 ́ | 5 ́-CAATGAAAGGAGGTAGACATAAACAGT – 3 ́ | ||
BDTG 33 | SNAP | 60.2 | 2 | 7395 | 7610 | 5 ́-ACTGTTTATGTCTACCTCCTTTCATTG – 3 ́ | 5 ́-AATAGATTTGCTACGGTCGAACA – 3 ́ | |
BDTG 34 | Kinase | 59.7 | 3 | 7718 | 8081 | 5 ́-TTTGTTATGGAATTCTAGTGTTGGAA – 3 ́ | 5 ́-CCAACATAACATCAGCATGTCTC – 3 ́ |