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Table 6 Comparison of genotypes identified by SSCP and pyrosequencing

From: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing

SSCP PYROSEQUENCING
Genotype 1 2 3 4 5 6 7 8 9
1. WT/WT (n = 3) 2 (66.7%) 0.0 0.0 0.0 0.0 0.0 1 (33.3%) 0.0 0.0
2. WT/MT (n = 11) 0.0 9 (81.8%) 1 (9.1%) 0.0 0.0 0.0 1 (9.1%) 0.0 0.0
3. MT/MT (n = 1) 0.0 0.0 1 (100%) 0.0 0.0 0.0 0.0 0.0 0.0
n = 15 n = 15
  1. For SSCP EAAT2F and EAAT2Pyro primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer. The genotypes that were correctly identified by both methods are indicated in bold.
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