BMC Genomic Data

Table 6 Comparison of genotypes identified by SSCP and pyrosequencing

From: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing

SSCP	PYROSEQUENCING
Genotype	1	2	3	4	5	6	7	8	9
1. WT/WT (n = 3)	2 (66.7%)	0.0	0.0	0.0	0.0	0.0	1 (33.3%)	0.0	0.0
2. WT/MT (n = 11)	0.0	9 (81.8%)	1 (9.1%)	0.0	0.0	0.0	1 (9.1%)	0.0	0.0
3. MT/MT (n = 1)	0.0	0.0	1 (100%)	0.0	0.0	0.0	0.0	0.0	0.0
n = 15	n = 15

For SSCP EAAT2F and EAAT2Pyro primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer. The genotypes that were correctly identified by both methods are indicated in bold.

Back to article page

ISSN: 2730-6844

Contact us

General enquiries: journalsubmissions@springernature.com