Skip to main content

Table 5 Comparison of genotypes identified by SSCP and pyrosequencing

From: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing

SSCP Pyprosequencing
Genotype 1 2 3 4 5 6 7 8 9
1. WT/WT (n = 51) 38 (74.5%) 1 (1.9%) 0.0 0.0 4 (7.8%) 0.0 8 (15.6%) 0.0 0.0
2. WT/MT (n = 92) 0.0 88 (95.6%) 2 (2.2%) 0.0 0.0 0.0 0.0 2 (2.2%) 0.0
3. MT/MT (n = 39) 0.0 6 (15.3%) 27 (69.2%) 6 (15.4%) 0.0 0.0 0.0 0.0 0.0
4. (n = 1) 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0
n = 183 n = 183
  1. For the SSCP EAAT2F and EAAT2R primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer (Figure 1 and Table 1). The genotypes that were correctly identified by both methods are indicated in bold. The genotype numbers indicated under pyrosequencing corresponds to those used in Table 2.
Back to article page

BMC Genomic Data

ISSN: 2730-6844

Contact us