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Table 5 Comparison of genotypes identified by SSCP and pyrosequencing

From: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing

SSCP Pyprosequencing
Genotype 1 2 3 4 5 6 7 8 9
1. WT/WT (n = 51) 38 (74.5%) 1 (1.9%) 0.0 0.0 4 (7.8%) 0.0 8 (15.6%) 0.0 0.0
2. WT/MT (n = 92) 0.0 88 (95.6%) 2 (2.2%) 0.0 0.0 0.0 0.0 2 (2.2%) 0.0
3. MT/MT (n = 39) 0.0 6 (15.3%) 27 (69.2%) 6 (15.4%) 0.0 0.0 0.0 0.0 0.0
4. (n = 1) 0.0 0.0 0.0 1.0 0.0 0.0 0.0 0.0 0.0
n = 183 n = 183
  1. For the SSCP EAAT2F and EAAT2R primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer (Figure 1 and Table 1). The genotypes that were correctly identified by both methods are indicated in bold. The genotype numbers indicated under pyrosequencing corresponds to those used in Table 2.
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BMC Genomic Data

ISSN: 2730-6844

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