SSCP
|
Pyprosequencing
|
---|
Genotype
|
1
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
9
|
---|
1. WT/WT (n = 51)
|
38 (74.5%)
|
1 (1.9%)
|
0.0
|
0.0
|
4 (7.8%)
|
0.0
|
8 (15.6%)
|
0.0
|
0.0
|
2. WT/MT (n = 92)
|
0.0
|
88 (95.6%)
|
2 (2.2%)
|
0.0
|
0.0
|
0.0
|
0.0
|
2 (2.2%)
|
0.0
|
3. MT/MT (n = 39)
|
0.0
|
6 (15.3%)
|
27 (69.2%)
|
6 (15.4%)
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
4. (n = 1)
|
0.0
|
0.0
|
0.0
|
1.0
|
0.0
|
0.0
|
0.0
|
0.0
|
0.0
|
n = 183
|
n = 183
|
- For the SSCP EAAT2F and EAAT2R primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer (Figure 1 and Table 1). The genotypes that were correctly identified by both methods are indicated in bold. The genotype numbers indicated under pyrosequencing corresponds to those used in Table 2.