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Table 5 Comparison of genotypes identified by SSCP and pyrosequencing

From: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing

SSCP

Pyprosequencing

Genotype

1

2

3

4

5

6

7

8

9

1. WT/WT (n = 51)

38 (74.5%)

1 (1.9%)

0.0

0.0

4 (7.8%)

0.0

8 (15.6%)

0.0

0.0

2. WT/MT (n = 92)

0.0

88 (95.6%)

2 (2.2%)

0.0

0.0

0.0

0.0

2 (2.2%)

0.0

3. MT/MT (n = 39)

0.0

6 (15.3%)

27 (69.2%)

6 (15.4%)

0.0

0.0

0.0

0.0

0.0

4. (n = 1)

0.0

0.0

0.0

1.0

0.0

0.0

0.0

0.0

0.0

n = 183

n = 183

  1. For the SSCP EAAT2F and EAAT2R primers were used and pyrosequencing was done with EAAT2PyroSeq1 primer (Figure 1 and Table 1). The genotypes that were correctly identified by both methods are indicated in bold. The genotype numbers indicated under pyrosequencing corresponds to those used in Table 2.