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Table 1 PCR primer sequences for methylation analysis of PTPRO

From: Aberrant PTPRO methylation in tumor tissues as a potential biomarker that predicts clinical outcomes in breast cancer patients

Primers Sequences Product size
PTPRO-forward 5′-CTCCACCCAAATCACTCTTCGCAG-3′ 268 bp
PTPRO-reverse 5′-ACCATTGTTGAGACGGCTATGAACG-3′
18 s rRNA-forward 5′-TCAAGAACGAAAGTCGGAGG-3′ 110 bp
18 s rRNA- reverse 5′-GGACATCTAAGGGCATCACA-3′
MSP-methylated-forward 5′-CGTTTTTGGAGGATTTCGGGC-3′ 170 bp
MSP-methylated- reverse 5′-AAAACACGACTACGCTAACG-3′
MSP-unmethylated-forward 5′-ATGTTTTTGGAGGATTTTGGGT-3′ 201 bp
MSP-unmethylated- reverse 5′-ATACCCCATCACTACACAAACA-3′  
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