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Table 1 Summary of reduced-lifespan genes

From: A gain-of-function screen to identify genes that reduce lifespan in the adult of Drosophila melanogaster

Reduced-lifespan gene

GS number of 1st positive lines

HS/no-HSc

p35/GFPd

Transportin

11030a, 13069b

1.9/63.0 (3.02%)

3.0/4.1 (−)

Dream

16231a, b

1.3/34.6 (3.76%)

41.6/1.0 (+)

CG10277

11124a, b, 15951

1.9/50.0 (3.80%)

1.0/2.0 (−)

Hepatocyte nuclear factor 4

10535a, b

1.9/48.6 (3.91%)

2.9/41.3 (−)

Calpain-A

11846a, b

2.1/46.0 (4.57%)

ND

Transfer RNA:ile:49Fb

14941a, b

3.0/65.1 (4.61%)

54.9/2.0 (+)

Combgap

11121a, b, 14406

2.0/43.0 (4.65%)

2.0/3.0 (−)

trpγ

16894a, b

1.8/36.8 (4.89%)

1.1/1.0 (−)

Apontic

15217a, b

2.2/44.8 (4.91%)

3.0/3.0 (−)

Cadmus

14710a, b

3.0/61.0 (4.92%)

2.7/3.8 (−)

PolyA-binding protein

13841, 15168a, b

2.4/46.7 (5.14%)

21.3/5.2 (+)

Embryonic lethal abnormal vision

5211a, b

2.6/43.6 (5.96%)

21.7/4.1 (+)

CG3363

11052a, b, 11427, 13243

3.0/49.0 (6.12%)

8.4/7.0 (−)

Stonewall

8015a, b, 16462, 17037

3.2/52.0 (6.15%)

6.8/21.2 (−)

Degringolade

3250, 15270a, b

3.0/48.6 (6.17%)

11.9/4.1 (−)

Atlastin

7264, 11283, 17467a

2.5/36.0 (6.94%)

2.0/1.7 (−)

Chromator

8043, 16669a, b, 17953

2.0/27.8 (7.19%)

3.3/3.1 (−)

Slipper

7470a, b

1.3/16.0 (8.13%)

31.9/1.0 (+)

Polo

16634a, b, 16673, 20105

3.9/44.8 (8.71%)

13.9/28.0 (−)

Without children

10711a, b

2.8/32.0 (8.75%)

8.1/2.1 (+)

Heterogeneous nuclear ribonucleoprotein at 27C

10478, 11696, 13072a, b, 13193, 14409, 14755, 16784, 16954

3.4/38.0 (8.95%)

22.3/36.5 (−)

High mobility group protein D

9106, 9272, 9689b, 10504, 10665a, 12910, 17197

2.6/25.0 (10.40%)

3.0/2.0 (−)

Fmr1

11128b, 11947a

5.0/45.0 (11.11%)

11.4/5.4 (−)

Peroxin 16

12435a, b, 14333, 15386

3.6/31.0 (11.61%)

17.4/22.1 (−)

Pipsqueak

12449a, b

6.7/51.1 (13.11%)

9.9/41.1 (−)

CG8290

12665a, b

4.7/34.9 (13.47%)

34.8/7.8 (+)

Vacuolar H + ATPase subunit 100-2

10911a, b, 14367

7.1/50.4 (14.09%)

2.8/31.6 (−)

CG10321

10668a, b

3.9/27.0 (14.44%)

3.2/4.4 (−)

Roadkill

14361, 14844, 15232, 15233a, b

6.3/41.8 (15.07%)

3.0/6.6 (−)

Lamin

16890a, b

4.5/28.3 (15.90%)

3.9/3.4 (−)

Elfless

15946a, b

3.3/20.0 (16.50%)

4.0/9.0 (−)

CG16779

9601a, b

4.4/26.0 (16.92%)

36.5/32.5 (−)

Elongation factor Tu mitochondrial

11862a, b

8.6/42.2 (20.38%)

14.9/20.1 (−)

CG11819

5065a, b

6.1/29.0 (21.03%)

22.7/14.8 (−)

Autophagy-specific gene 1

15847a, b

12.6/57.8 (21.80%)

21.8/5.0 (+)

CG8032

5196a, b

2.8/12.1 (23.14%)

46.9/8.3 (+)

N-methyl-D-aspartate receptor-associated protein

16440a, b

3.1/13.3 (23.31%)

2.8/17.7 (−)

Src oncogene at 42A

11022, 11049a, b

2.6/11.0 (23.64%)

4.5/5.4 (−)

CG30482

9799a, b

6.8/26.3 (25.86%)

5.0/28.5 (−)

Average

 

4.0/38.5 (10.40%)

13.5/11.6

  1. aRepresentative GS lines used for second screen. The mean longevities are listed on the third column (HS/no-HS).
  2. bGS lines used for p35 assay.
  3. cMean longevity (days) of heat-shocked (HS)/not-heat-shocked flies (no-HS).
  4. dMean longevity (days) of flies coexpressing p35 (p35)/or GFP (GFP). (+) indicates longevity extended more than three times by p35 as compared to GFP coexpression.