Figure 2From: A gain-of-function screen to identify genes that reduce lifespan in the adult of Drosophila melanogasterComparison of the mean longevity of F1 progeny with or without heat shock. (A and B) The mean longevity of F1 progeny carrying each GS vector and hs-GAL4 was measured with or without heat shock. The numbers of GS lines with the indicated mean longevity are shown for each 2-day period. White and black bars correspond to the number of GS lines treated without (−) or with (+) heat shock, respectively. (A) Mean longevity averages for 122 randomly selected GS lines: the average mean longevity of flies treated without or with heat-shock was 56.6 and 55.2 days, respectively. (B) GS lines misexpressing reduced-lifespan genes, identified from the screen. The average of the mean longevities of flies that were or were not heat-shocked was 3.7 and 38.5 days, respectively. (C and D) Box-and-whisker plots of the mean longevity averages for 122 randomly selected GS lines (C) and lines with reduced-lifespan genes (D), with (+) or without (−) heat shock (hs). Whiskers and outliers were determined by Tukey’s method. Significance indicated (**) is P < 0.001 (unpaired t-test). NS: not significant.Back to article page