Comparison of the mean longevity of F1 progeny with or without heat shock. (A and B) The mean longevity of F1 progeny carrying each GS vector and hs-GAL4 was measured with or without heat shock. The numbers of GS lines with the indicated mean longevity are shown for each 2-day period. White and black bars correspond to the number of GS lines treated without (−) or with (+) heat shock, respectively. (A) Mean longevity averages for 122 randomly selected GS lines: the average mean longevity of flies treated without or with heat-shock was 56.6 and 55.2 days, respectively. (B) GS lines misexpressing reduced-lifespan genes, identified from the screen. The average of the mean longevities of flies that were or were not heat-shocked was 3.7 and 38.5 days, respectively. (C and D) Box-and-whisker plots of the mean longevity averages for 122 randomly selected GS lines (C) and lines with reduced-lifespan genes (D), with (+) or without (−) heat shock (hs). Whiskers and outliers were determined by Tukey’s method. Significance indicated (**) is P < 0.001 (unpaired t-test). NS: not significant.