Table 2 Possible changes in MSAP MH profiles from Figure 1 and their interpretation
From: How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles?
Plants | |||
|---|---|---|---|
1MH1 → MH2 | methylation change | MSAP pattern (Figure 1) | 7interpretation |
2(-, -) → (+, +) | 3s(mCmC → CC) | 4S5(4 → 1) | ambiguous |
6s(mCmC → CC) and l(CmC → CC) | S(5 → 1) | ||
s(CC → mCmC) | L(1 → 4) | ||
s(CmC → mCmC) and l(CmC → CC) | L(3 → 4) | ||
(-, -) → (+, -) | s(mCmC → CmC) | S(4 → 2) or S(5 → 3) | ambiguous |
s(mCmC → CmC) and l(CC → CmC) | S(4 → 3) | ||
s(mCmC → CmC) and l(CmC → CC) | S(5 → 2) | ||
s(CmC → mCmC) | L(3 → 5) | ||
s(CC → mCmC) and l(CC → CmC) | L(1 → 5) | ||
(-, -) → (-, +) | l(CmC → CC) | L(3 → 2) | ambiguous |
s(CC → CmC) | L(1 → 2) | ||
(+, +) → (-, -) | s(CC → mCmC) | S(1 → 4) | ambiguous |
s(CC → mCmC) and l(CC → CmC) | S(1 → 5) | ||
s(mCmC → CC) | L(4 → 1) | ||
s(mCmC → CmC) and l(CC → CmC) | L(4 → 3) | ||
(+, +) → (+, -) | s(CC → CmC) | S(1 → 2) | methylation |
s(CC → CmC) and l(CC → CmC) | S(1 → 3) | ||
l(CC → CmC) | L(4 → 5) | ||
(+, +) → (-, +) | s(mCmC → CmC) | L(4 → 2) | hypomethylation |
(+, -) → (-, -) | s(CmC → mCmC) | S(2 → 4) or S(3 → 5) | ambiguous |
s(CmC → mCmC) and l(CC → CmC) | S(2 → 5) | ||
s(CmC → mCmC) and l(CmC → CC) | S(3 → 4) | ||
s(mCmC → CmC) | L(5 → 3) | ||
s(mCmC → CC) and l(CmC → CC) | L(5 → 1) | ||
(+, -) → (+, +) | s(CmC → CC) | S(2 → 1) | hypomethylation |
s(CmC → CC) and l(CmC → CC) | S(3 → 1) | ||
l(CmC → CC) | L(5 → 4) | ||
(+, -) → (-, +) | s(mCmC → CmC) and l(CmC → CC) | L(5 → 2) | hypomethylation |
(-, +) → (-, -) | s(CmC → CC) | L(2 → 1) | ambiguous |
l(CC → CmC) | L(2 → 3) | ||
(-, +) → (+, +) | s(CmC → mCmC) | L(2 → 4) | methylation |
(-, +) → (+, -) | s(CmC → mCmC) and l(CC → CmC) | L(2 → 5) | methylation |
Vertebrates | |||
MH1 → MH2 | sequence change | MSAP pattern (Figure 1) | interpretation |
(-, -) → (+, +) | 8new CCGG | - | mutation |
(-, -) → (+, -) | new CmCGG | - | mut. + met. |
(-, -) → (-, +) | l(CmC → CC) | L(3 → 2) | ambiguous |
s(CC → CmC) | L(1 → 2) | ||
(+, +) → (-, -) | CCGG loss | - | mutation |
(+, +) → (+, -) | s(CC → CmC) | S(1 → 2) | methylation |
(+, +) → (-, +) | new internal CmCGG | - | mut. + met. |
(+, -) → (-, -) | CmCGG loss | - | mutation |
new internal CmCGG | - | ||
(+, -) → (+, +) | s(CmC → CC) | S(2 → 1) | hypomethylation |
s(CmC → CC) and l(CmC → CC) | S(3 → 1) | ||
(+, -) → (-, +) | (CmC → CC) and new int. CmCGG | - | mutation |
(-, +) → (-, -) | s(CmC → CC) | L(2 → 1) | ambiguous |
l(CC → CmC) | L(2 → 3) | ||
(-, +) → (+, +) | internal CmCGG loss | - | mutation |
(-, +) → (+, -) | (CC → CmC) and int. CmCGG loss | - | mutation |