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Figure 2 | BMC Genetics

Figure 2

From: How to interpret Methylation Sensitive Amplified Polymorphism (MSAP) profiles?

Figure 2

Verification of the internal CmCGG site in (-, +) MH Eco RI- Hpa II fragments by the addition of the M + H profile. Two different DNA molecules producing a (-, +) MH pattern for the long (L) fragment can contain either an internal CmCGG site (1) or hemimethylated mCCGG site (2). Combined digestion by both MspI and HpaII enzymes (M + H lanes) distinguish between the two molecules. Note that digested short (S) and long (L) DNA fragments may be amplified by different selective primers; if so, they are not visible together on one MSAP profile.

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