Diagrammatic illustration showing PCR strategy for full length amplification of 3.56 Kb unit of DYZ1 array. (A) Cartoon diagram of human Y chromosome with highlighted DYZ1 containing region in purple, (B) a single unit of DYZ1array is shown as a purple bar. Forward and reverse primers are shown above and below the bar. Similarly, amplification products and their corresponding sizes are shown below the bar. The amplified fragments were used for subsequent cloning and sequencing.