Difference in programmed cell death with the activation of Caspase-3 in the GER of R75W + mouse at P11. At P11, whole mount cochlear tissue of R75W + mice and the littermate controls was immunolabeled by anti-Cleaved-Caspase-3 (red, C-Casp3) with Phalloidin for F-actin (green) and DAPI for the nucleus (light green). Fifty slices of z-stack confocal images for the organ of Corti were collected at 0.5 μm intervals (total 25.5 μm depth from the surface of the organ of Corti). (A-D) The single image stacks for R75W + mouse (B, D) and the littermate controls (A, C) were constructed with LSM Image Browser (Zeiss). C-Casp3 and F-actin labelings are shown with (C, D) or without (A, B) nuclear labeling. (E-H) Three-dimensional images were constructed with the above confocal images of C-Casp3 and F-actin labeling with (G, H) or without (E, F) nuclear labeling. Bars indicate 20 μm.