Comparison of relative expression of SCNN1 gene family members vs β-actin measured by qPCR in different tissues. The kidney was collected from four normal 49-Week-old White Leghorn layers while all others tissues, breast muscle, liver, jejunum, ovary, magnum, isthmus, active uterus (during eggshell deposition) and quiescent uterus (no egg present at time of tissue collection) were collected from four normal 55-week-old White Leghorn layers. The y-axis indicates the relative expression level of each SCNN1 family member. Different letters (A,B,C, D) represent significant difference (P < 0.05). Panel (a), Panel (b), Panel (c) and Panel (d) represents the expression of the SCNN1a, SCNN1b, SCNN1g and SCNN1d, respectively.