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Table 6 SNP type and distributions along with SNP comparisons of sequences found within and between species (homologous sequence comparisons) using SNP_Finder_Plus (8X min. coverage, 30% min. minor allele, 90% min. identity)

From: Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing

Species assembly

SNP

Average coverage

SNPs/assembly length1

SNP distribution

A↔C

A↔G

A↔T

C↔G

C↔T

G↔T

P. cyananthus

2,493

16.4

0.000539 (~1/1855 bp)

10.7%

29.5%

13.9%

4.3%

30.2%

9.5%

P. dissectus

737

14.3

0.000280 (1/3568 bp)

9.8%

30.7%

15.6%

4.6%

27.4%

9.8%

P. davidsonii

713

14.4

0.000300 (~1/3333 bp)

11.9%

26.4%

15.2%

3.9%

28.3%

11.8%

P. fruticosus

615

12.4

0.000265 (~1/3777 bp)

11.7%

27.2%

17.9%

4.2%

25.4%

12.0%

Homologous sequence comparisons

P. cyananthus × P. dissectus

3,253

10.6

0.009610 (~1/104 bp)

11.7%

27.5%

16.0%

7.1%

27.1%

10.6%

P. cyananthus × P. davidsonii

1,958

10.7

0.008062 (~1/124 bp)

11.1%

27.6%

15.8%

7.1%

28.5%

9.9%

P. cyananthus × P. fruticosus

2,015

10.6

0.008367 (~1/119 bp)

10.6%

27.2%

16.7%

6.8%

28.7%

10.1%

P. dissectus × P. davidsonii

2,348

10.8

0.015605 (~1/64 bp)

12.6%

26.7%

15.5%

7.5%

27.3%

10.4%

P. dissectus × P. fruticosus

2,133

10.0

0.011991 (~1/83 bp)

12.0%

26.4%

16.5%

7.6%

27.2%

10.4%

P. davidsonii × P. fruticosus

2,156

10.1

0.008404 (~1/119 bp)

12.8%

28.2%

14.5%

7.2%

27.2%

10.1%

  1. 1 Assembly length is bases shared between assemblies (see Table 4).