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Table 4 Summary data from 454-pyrosequencing and Newbler de-novo assembly (v.2.0.01) of Penstemon cyananthus , P. dissectus , P. davidsonii , and P. fruticosus

From: Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing

Assembly Genome size (Mbp)1 GC content Reads Bases2 % Reads assembled % Bases assembled Contigs created Bases in assembly % Genome represented Average coverage Bases shared between assemblies
P. cyananthus 893 36.4% 199,329 87,753,792 53.1% 50.0% 9,714 4,623,755 0.5% 7.7X  
P. dissectus 462 34.5% 98,868 43,304,550 52.8% 50.9% 5,364 2,629,819 0.6% 8.2X  
P. davidsonii 483 35.3% 103,963 45,599,742 45.8% 43.5% 4,882 2,376,141 0.5% 9.1X  
P. fruticosus 476 35.2% 113,146 49,786,980 41.0% 38.8% 4,777 2,322,606 0.5% 8.9X  
P. cyananthus × P. dissectus    298,197 131,058,342 53.0% 50.1% 14,523 6,915,079    338,495
P. cyananthus × P. davidsonii    303,292 133,353,534 49.9% 46.9% 14,254 6,757,023    242,873
P. cyananthus × P. fruticosus    312,475 137,540,772 47.8% 44.9% 14,134 6,705,536    240,825
P. dissectus × P. davidsonii    202,831 88,904,292 48.3% 46.1% 10,053 4,855,491    150,469
P. dissectus × P. fruticosus    212,014 93,091,530 45.7% 43.5% 9,873 4,774,539    177,886
P. davidsonii × P. fruticosus    217,109 95,386,722 44.0% 41.7% 9,184 4,442,194    256,553
Full Penstemon Assembly    730,215 265,987,500 47.9% 46.4% 44,966 16,363,589    
  1. 1 The diploid (2n = 2x = 16) genome size as reported by Broderick et al. [5].
  2. 2 Bases denotes the total number of bases used to create the assembly and not the total number of bases sequenced.