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Table 1 Penstemon taxa (with collection counties) utilized in the 12 marker analysis with respective marker sizes

From: Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing

Species County1 Marker sizes in bp
PS004 PS011 PS012 PS014 PS017 PS032 PS034 PS035 PS048 PS052 PS053 PS075
Subgenus Dasanthera              
P. davidsonii Purchased2 460 500 360 370 700 370 320, 950 520 440 220 320 140
P. fruticosus v. fruticosus Purchased 460 500 360 410 700 340 360 520 420 220 320 140
P. montanus v. montanus Utah 480 430 390 370 450 310 340, 310 470 430 200 390 115
Subgenus Dissecti              
P. dissectus Purchased 440 860 370 380 750 370 320 920 380 220 320, 450 140
Subgenus Habroanthus              
P. ammophilus Kane 480 800 400 430 470 300 1250, 340 470 420 230 360 125
P. barbatus v. torreyi Garfield 420 800 400 490 500 320 340 500 410 200 360 110
P. barbatus v. trichander San Juan 650, 480 850 420 500, 490 520 310 310 500 450, 410 200 370 130
P. comarrhenus Garfield 650, 480 850 420 490 470 330, 310 310 500 430 200 360 125
P. compactus Cache 440 850 400 500 490 300 300 480 410 210 390, 360 125
P. cyananthus v. cyananthus Wasatch 420 860 400 410 750 370 310, 340 630 420 220 160, 320 160
P. cyananthus v. subglaber Box Elder 440, 420 850 400 490, 470 500, 450 340, 310 310, 280 520 410 210 360 120
P. cyanocaulis Emery 440 310 420 490, 470 520 330, 320 320 480 420 210 350 120
P. eatonii v. eatonii Utah 420 800 420 490 450 320 300 500 NM3 210 350 135, 125
P. eatonii v. undosus Washington 420 850 420 470 420 320 290 650, 500 410 210 340 125
P. fremontii Uintah 480 850 400 430 490 320, 310 340 500 420 220 370 130
P. gibbensii Daggett 480, 440 850 420 490 420 320 300 480 430 220 360 130
P. idahoensis Box Elder 440 800 400 410 470 310 340 500 430 250 340 130
P. laevis Kane 440 850 400 470 470 310 350, 320 500 420 220 360 125
P. leiophyllus v. leiophyllus Iron 480 850, 490 420 430 450 310 340 480 430 220 350 120
P. longiflorus Beaver 440 800 420, 400 470 470, 450 330, 310 310 500 450 230 350, 220 125
P. navajoa San Juan 480 800 400 490 550 330, 300 360, 340 500 450 230 410 135, 130
P. parvus Garfield 480 850 450 500, 490 490 320 300 500 430 210 380, 360 130
P. pseudoputus Garfield 480 800 420, 400 430 490, 420 320 340 480 450 230, 220 350 130
P. scariosus v. albifluvis Uintah 440 850 400 490 490 310 320 480 410 210 370 115
P. scariosus v. cyanomontanus Uintah 440 850 400 490 490 330 310 500 420 210 360 115
P. scariosus v. garrettii Duchesne 490, 480 850 420 430 490 320 420, 340 520 430 230 360 125
P. scariosus v. scariosus Sevier 480 1500, 1300 400 470 520 340, 310 310 500 430 210 360 130
P. speciosus Box Elder 440 800 420 500, 490 490 320 340, 310 520 310 210 360 120
P. strictiformis San Juan 480 850 400 470 500, 470 370, 310 350 500 410 220 370 125
P. strictus Wasatch 480 850 400 410 450 310 350 520, 500 430 230 340 110
P. subglaber Sevier 480 850 420, 400 470 490 310 350 500 430 220 350 115
P. tidestromii Juab 390 850 420 470 490 310 300 480 400 190 360 140, 120
P. uintahensis Duchesne 480 850 420 490 450 340 300 520 410 220 380 120
P. wardii Sevier 480 800 420 430 490, 450 310 310 520 420 220 340 135, 120
Subgenus Penstemon              
P. abietinus Sevier 440 AD4 390 400 520 320 340 500 430 230 350 125
P. acaulis Daggett 570 490 420 430 470 320 350 480 420 220 340 120
P. ambiguus v. laevissimus Washington 520 850 390 490 470 320 1250, 340 500 400 220 AD 120
P. angustifolius v. dulcis Millard 440 850, 600 400 490 520 370, 150 310 520 420 220 360 125
P. angustifolius v. venosus San Juan 480 310 390 470 470 320, 150 340 550 450 220 360 135
P. angustifolius v. vernalensis Daggett 480 800 390 430 470 370, 150 350 500 420 220 380 125
P. atwoodii Kane 440 800 420 490, 390 470 300 310 480 400 180 360, 280 115
P. bracteatus Garfield 440 850 400 500 AD 330, 310 320 520 420 230 380 125
P. breviculus San Juan 650, 480 190 400 AD 470 500, 220 320 480 430 210 350 125
P. caespitosus v. caespitosus Uintah 440 850 390 390 490 320 NM 190 NM 210 390, 370 115
P. caespitosus v. desertipicti Washington 440 230 390 470, 370 470, 360 330 350 1000, 300 430 210 400, 380 130
P. caespitosus v. perbrevis Wasatch 420 490 390 430, 400 470 320 350 520 380 220 340 120
P. carnosus Emery 440 850 420 490 490 330, 300 310 500 430 220 350 130, 120
P. concinnus Beaver 440 800 420 430, 400 500 480 350 480 420 190 700, 360 120
P. confusus Washington 480 850 420 490 520 300 320 480 450 220 350 125
P. crandallii v. atratus San Juan 420 490 390 500 450 370 320 280 400 190 350 120
P. crandallii v. crandallii San Juan 420 340, 190 390 500 450 370, 340 310 280 380 190 350 115
P. deustus v. pedicellatus Teton 420 850 420 430 550 340 320 550 340 230 370 130
P. dolius v. dolius Millard NM 710 400 400 490, 320 530, 300 320 480 450, 420 180 340 105
P. dolius v. duchesnensis Duchesne 420 AD 420 400 500 340 320 480 410 180 360, 340 140, 120
P. eriantherus v. cleburnei Daggett 420 850 420 410 450 480 300 500 490, 420 190 390, 360 140, 130
P. flowersii Uintah 480 AD 420, 400 490, 430 470 300 350 520 420 220 360 125
P. franklinii Iron 480 800 400 430 470 320, 300 350 520 420 240 380 125
P. goodrichii Uintah 420 650 390 400 490 480 310 480 400 200 370, 350 135
P. grahamii Uintah 420 850 400 390 470 530, 320 350 500 420 230 500, 370 120
P. humilis v. brevifolius Cache 390 850 370 500 450 340, 320 320 480 500 220 280 115
P. humilis v. humilis Box Elder 420 850 390 410 520 330, 310 360 500 500, 470 220 350 120
P. humilis v. obtusifolius Washington 420 800 390 520, 490 AD 330 340 480 470 200 350 120
P. immanifestus Millard 480 710 420 490 380 300 320 480 410, 380 220 400, 360 120
P. lentus v. albiflorus San Juan NM 430 390 430 450 320 300 500 400 210 470, 370 140
P. lentus v. lentus San Juan 480 850 400 430 470 300 310 500 410 210 400, 370 145
P. linarioides v. sileri Washington 420 850 370 490, 390 470 330, 310 350 470 400 210 370 125
P. marcusii Emery 390 800 450 370 490 310 340, 320 500 NM 200 390, 360 120
P. moffatii Grand 390 800 420 390 490 330 340 480 430 290, 200 380, 350 140
P. nanus Millard 480 800 420 390 470 280 320 470 NM 180 360 120
P. ophianthus Sevier 520 850 420 370 900, 750 330, 310 310 480 420 190 AD 115
P. pachyphyllus v. congestus Kane 480 850 400 430 470, 380 320 310 520 410 250 370 170
P. pachyphyllus v. mucronatus Daggett 440 800 390, 370 430 500 320 300, 280 520, 500 430 220 350 120
P. pachyphyllus v. pachyphyllus Duchesne 480 850 390 410 490 370, 330 340, 240 400, 190 500, 430 290, 230 380, 220 125
P. palmeri v. palmeri Washington 440 850 400 500, 490 520, 490 330 310 500 430 210 380 125
P. petiolatus Washington 420 1000 400 500, 490 500 330 300 480 420 210 380 145
P. pinorum Washington 480 800 420 610 500 480 310 480 470 200 390 125
P. procerus v. aberrans Garfield 440 1000, 850 450, 370 520 520 330 360 480 410 220 370 115
P. procerus v. procerus Iron 420 850, 550 370 490 470 340, 310 360 470 470 220 340 120
P. radicosus Daggett 420 AD 420 490 470 330, 310 310 500 450 200 360 125
P. rydbergii v. aggregatus Box Elder 420 850 400 520 500 340 360 520 470 210 380 115
P. rydbergii v. rydbergii Rich 420 710 400 520 500 370 320 500 470, 430 AD 390 115
P. thompsoniae Kane 420 AD 370 500 450 340, 320 340 500 410 220 390, 370 130
P. tusharensis Beaver 420 1300, 230 370 430 450 320 320 500, 300 410 230 340 120
P. utahensis San Juan 480 410 420 430 490 300 310 500 410 220 370 125
P. watsonii Sevier 420 AD 370 490 470 320 350 480 490 220 350 120
P. whippleanus Iron 420 800 400 370 450 310 350 500 430 210 370 105
P. yampaensis Daggett 570 710 400 430, 390 490 500, 320 310 480 410 260, 230 340 120
Subgenus Saccanthera              
P. leonardii v. higginsii Washington 390 1300 420, 400 490, 430 550, 520 320 310 480 470 250 AD 125
P. leonardii v. leonardii Utah 440 800 420 430 490 320 340, 320 480 500 240 370 120
P. leonardii v. patricus Tooele 440 850 370 470 AD 370 310 550, 520 470 230 380 115
P. platyphyllus Salt Lake 420 800 400 430 470 330 310 520 430 240 AD 135
P. rostriflorus Washington 420 1100, 430 400 410 420 320, 300 290 500 490, 430 470 370 120
P. sepalulus Utah 420 800 400 470 450 330 310 500 430 230 390 130
Total unique molecular weight bands 9 18 6 10 14 12 12 13 12 11 17 11
Total pairs of dual molecular weight bands 6 7 7 16 11 28 14 7 8 4 20 7
Total monomorphic markers   85 80 86 76 80 65 78 86 81 88 69 86
Total NM   2 0 0 0 0 0 1 0 4 0 0 0
Total AD   0 6 0 1 2 0 0 0 0 1 4 0
  1. 1 All counties are in Utah except Teton, Co. which is in Wyoming.
  2. 2 Purchased = P. davidsonii and P. fruticosus were purchased from nurseries in Utah Co., Utah while P. dissectus was purchased from a nursery in Aiken Co., South Carolina.
  3. 3 NM = no marker.
  4. 4 AD = ambiguous data (usually multiple bands and or smearing).