Effect of BTNL8-BTNL3_del on BTNL9 level in LCLs. BTNL9 mRNA and protein levels were measured by RT-qPCR and western-blot analysis, respectively. (A) For each genotype expression-level of five different samples were measured. Samples containing at least one deleted allele show significant reduced amount in BTNL9 mRNA level. (B) Representative western-blot shows expression of BTNL9 in cell homozygous for the deletion versus cells homozygous for the non-deleted allele. The band intensities were normalized to tubulin expression.