Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesis

Table 1 Primers in this study

	Gene	GenBank ID	Primer sequence (5′-3′)	Annealing temp (°C)	Product size (bp)	Application
P1	Bvh	AF541971	F: GAAGATTGGGAAGCAGAAA	58	1457	cDNA clone
P1	Bvh	AF541971	R: CTGACGCTGTTCCTTTGAT	58	1457	cDNA clone
P2	Bvh	AF541971	F: AACAGGCATAAACTTTGACA	59	1525	cDNA clone
P2	Bvh	AF541971	R: GGGTGGGAGTAAGAACAGA	59	1525	cDNA clone
P3	Bvh-FL	AF541971	F: TGCCTCTGGGAGGAGTTTGG	60	294	Real-time-PCR
P3	Bvh-FL	AF541971	R: GGCAACCTCGGAAACTACC	60	294	Real-time-PCR
P4	β-actin	NM_173979	F: CGGACTGTTAGCTGCGTTAC	60	164	Real-time PCR
P4	β-actin	NM_173979	R: CACCTTCACCGTTCCAGTT	60	164	Real-time PCR
P5	Bvh	JX437185	F: AAG GTA AGA ATC TCC CAC TC	58	337	PCR-RFLP
P5	Bvh	JX437185	R: GTC CTT GGC ACT TTC TAC AC	58	337	PCR-RFLP
P6	Bvh-V4	JX437188	F: AGATCCTGGTTTTTCAAATAAC	60	193	Real-time PCR
P6	Bvh-V4	JX437188	R: GCAACCTCGGAAACTACCT	60	193	Real-time PCR
P7	Bvh-V45	JX437189	F: CGTCAGATCCTGGTGAGTCT	60	83	Real-time PCR
P7	Bvh-V45	JX437189	R: TCTGTTTCCAAAACTCTTT	60	83	Real-time PCR
P8	Bvh	NW_003104511	F: GGATTGTAGTAGGTAAAAAAAGGAGA	53	346	Bisulfite sequencing PCR
P8	Bvh	NW_003104511	R: TCCAACAACAAATAACACCAA	53	346	Bisulfite sequencing PCR

ISSN: 2730-6844