Figure 6From: Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesisPromoter methylation statues of Bvh gene in testis of cattle, yak and cattle-yak hybrid. A. BSP sequence and distribution of CpG sites in Bvh promoter of cattle, yak and cattle-yak hybrid. Underline means CpG site. Arrow means primers for BSP. Dashed line indicate core promoter. Nucleotide numbering is relative to +1 at the initiating ATG codon. B. The genomic organization of the cattle Bvh gene. Numbered vertical lines indicate exons. Hatched boxes indicate BSP-amplyfied region. C. The CpG sites of BSP-amplified region. Vertical lines indicate 20 CpG site. D. Bisulfite sequencing results for cattle, yak and cattle-yak hybrid. Each line represents an individual bacterial clone which was sequenced. Black dots indicate methylated CpG sites, open circles indicate unmethylated CpG sites.Back to article page