PCR-RFLP assay of Bvh nt1202 site. A. Gel photograph of 337 bp fragments of Bvh. Line1-3 were cattle, yak and cattle-yak hybrid, respectively. Molecular marker was DL500. B. Restriction patterns of 337 bp fragments of Bvh digesting with Nde I enzyme on 1.5% agarose gel. Line 1 and 2 was genotype TT (337 bp). Line 3 and 4 was genotype CC (232 bp + 105 bp 337 bp). Line 5 and 6 was genotype TC (337 bp + 232 bp + 105 bp). C. Sequence of different genotypes at the nt1202 of Bvh gene. Arrow denotes substitution position. Red arrow denotes genotype TT, Blue arrow denotes genotype CC, Red and blue arrow denotes genotype TC.