Assessment of MspJI-seq technology. (A) Pearson correlation analysis of detected mCNNR number on enriched peaks of sequencing reads between two MspJI-seq replicates. (B) Pearson correlation analysis of mCNNR depth (methylation level) of enriched peaks between two MspJI-seq replicates. (C) Venn diagram indicating the overlap of YNmCGNR sites in enriched peaks detected in two MspJI-seq replicates and WGBS data. The specificity and sensitivity of MspJI method are calculated as the ratio of YNmCGNR sites commonly determined by MspJI-seq and WGBS to the total YNmCGNR sites determined by MspJI-seq or by WGBS, respectively. The two data sets of MspJI-seq replicates were counted separately for the two pairs of specificity and sensitivity. (D) The comparison of relative methylation levels on randomly chosen genomic regions (50 bp intervals) between two replicates of MspJI-seq and WGBS data.