Features of sequenced reads in MspJI-seq. (A) Distribution of all mapped reads on Arabidopsis genome. (B-E) Logos of insert fragments in length of 30 bp to 33 bp. Sequence characteristics were investigated by randomly extracting 1000 insert fragments in certain length from the uniquely mapped reads. Logos of 30 bp, 31 bp and 32 bp fragments (B, C and D) respectively present specific CHHG, CHG and CpG sites in the middle, and that of the 33 bp fragments (E) presents the CpG sites in a wobbling position. (F) Site categories in uniquely mapped reads with different length. The fragments in length of 26-33 bp mainly contain the symmetrical mCGNR and mCHGR sites, while fragments in other length mainly contain asymmetrical mCNNR sites. Results in Figure 1 were generated from the data of MspJI-seq replicate 1.