Table 2 Characteristics of the microsatellite loci from Paspalum atratum and P. notatum that were used in diversity analyses and cross-species amplification tests
Locus name | Repeat motif | Ta (°C) | Paspalumgenetic characterization | P.notatumgenetic characterization | Reference/GenBank accession no. | ||||
|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Product size (bp) | NM | PIC | Product size (bp) | NM | PIC | Â |
PA02-A5 | (GC)3GG(CT)4 | 60 | 122-142 | 9 | 0.59 | NA | NA | NA | 58 |
PA02-B1 | (CGGC)3 | 60 | 130-202 | 23 | 0.81 | 158-170 | 5 | 0.53 | 58 |
PA01-B7 | (GC)6(CA)2(GA)8CA(GA)9 | 60 | 185-310 | 32 | 0.89 | 249-300 | 7 | 0.68 | 58 |
PA01-C1 | (CA)3CG(CA)2CT(AGGA)3 | 58 | 231 | 10 | 0.25 | NA | NA | NA | 58 |
PA02-C3 | (CA)3CTGA(CA)4 | 60 | 222-400 | 13 | 0.78 | 240-266 | 3 | 0.09 | 58 |
PA01-C8 | (AT)2TTA(AT)2TTCA(GA)2 | 60 | 247-279 | 6 | 0.76 | NA | NA | NA | HM208140 |
PA01-E10 | (GGT)2 T(ACT)2 | 54 | 186-210 | 6 | 0.43 | NA | NA | NA | HM208141 |
PA02-F8 | (AC)4TA(AC)3 | TD | 166-200 | 6 | 0.42 | NA | NA | NA | 58 |
PA01- F10 | (CT)10 | 50 | 188-240 | 18 | 0.48 | 234-220 | 2 | 0.14 | 58 |
PN03-A6 | (GT)8 | 60 | 152-192 | 20 | 0.76 | 160-188 | 11 | 0.86 | 57 |
PN02-B5B | (TCA)4 | 60 | 152-234 | 24 | 0.79 | 180-204 | 4 | 0.58 | 57 |
PN03-C7 | (GT)6 | 50 | 133-177 | 20 | 0.83 | 159-165 | 3 | 0.58 | HQ585425 |
PA02-G11 | (CT)11 | TD | * | * | * | NA | NA | NA | HQ585425 |
PA02-H1 | (AC)3G(CA)3 | TD | * | * | * | NA | NA | NA | HM208150 |
PA02-H4 | (CA)2CT(CA)5 T(AC)2 | TD | * | * | * | NA | NA | NA | 58 |
PA02-H8 | (GT)3GCA(TG)3 | TD | * | * | * | NA | NA | NA | 58 |
PN03-A5 | (TG)8 | 60 | * | * | * | 249-287 | 4 | 0.54 | 57 |
PN03-F10 | (TG)7 | 60 | * | * | * | 165-177 | 7 | 0.78 | HQ585433 |
PN02-C6 | (TG)8 | 51.4 | * | * | * | 139-145 | 4 | 0.29 | HQ585422 |
PN02-F6A | (CT)11 | 60 | * | * | * | 154-218 | 22 | 0.89 | 57 |
PN03-D10 | (GT)11 | 50 | * | * | * | 280-300 | 7 | 0.66 | HQ585426 |
PN03-F2 | (AC)7 | 60 | * | * | * | 241-249 | 5 | 0.64 | 57 |
PN03-F9 | (CA)8 | 60 | * | * | * | 179-275 | 15 | 0.88 | HQ585432 |
PN02-A12 | (GA)3AT(GAA)3 | 60 | NA | NA | NA | 185-205 | 3 | 0.45 | 57 |
PN02-B3 | (GT)12 | 50 | NA | NA | NA | 139-157 | 11 | 0.84 | HQ585421 |
PN02-G10 | (CA)8 | 60 | NA | NA | NA | 189-219 | 7 | 0.83 | 57 |
PN02-G3 | (GT)9 | 60 | NA | NA | NA | 294-302 | 5 | 0.6 | HQ585423 |
PN02-G7 | (AC)7 | TD | NA | NA | NA | 118 | 7 | 0.74 | HQ585424 |
PN02-H7 | (AC)8 | 60 | NA | NA | NA | 194-204 | 4 | 0.63 | 57 |
PN03-D12 | (AG)10 | 50 | NA | NA | NA | 220-240 | 7 | 0.69 | HQ585427 |
PN03-E7 | (GT)9 | TD | NA | NA | NA | 298 | 7 | 0.74 | HQ585428 |
PN03-E9b | (AC)7 | 60 | NA | NA | NA | 168-186 | 6 | 0.59 | 57 |
PN03-F1 | (CA)7 | 50 | NA | NA | NA | 184-192 | 5 | 0.71 | HQ585429 |
PN03-F3 | (GT)8 | 60 | NA | NA | NA | 165-203 | 8 | 0.75 | HQ585430 |
PN03-F7 | (CA)10 | 50 | NA | NA | NA | 200-212 | 6 | 0.76 | HQ585431 |
PN03-G5 | (CA)7 | 60 | NA | NA | NA | 126-138 | 6 | 0.74 | HQ585434 |
PN03-G8 | (CA)7 | 60 | NA | NA | NA | 254-258 | 3 | 0.38 | 57 |
PN03-H10 | (CT)11 | 60 | NA | NA | NA | 222-272 | 13 | 0.86 | 57 |
PN03-H3 | (GA)22 | 51.4 | NA | NA | NA | 198-250 | 11 | 0.84 | HQ585435 |
Average | Â | Â | Â | 15.58 | 0.65 | Â | 7.52 | 0.64 | Â |