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Table 2 Characteristics of the microsatellite loci from Paspalum atratum and P. notatum that were used in diversity analyses and cross-species amplification tests

From: Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalumgenus

Locus name Repeat motif Ta (°C) Paspalumgenetic characterization P.notatumgenetic characterization Reference/GenBank accession no.
    Product size (bp) NM PIC Product size (bp) NM PIC  
PA02-A5 (GC)3GG(CT)4 60 122-142 9 0.59 NA NA NA 58
PA02-B1 (CGGC)3 60 130-202 23 0.81 158-170 5 0.53 58
PA01-B7 (GC)6(CA)2(GA)8CA(GA)9 60 185-310 32 0.89 249-300 7 0.68 58
PA01-C1 (CA)3CG(CA)2CT(AGGA)3 58 231 10 0.25 NA NA NA 58
PA02-C3 (CA)3CTGA(CA)4 60 222-400 13 0.78 240-266 3 0.09 58
PA01-C8 (AT)2TTA(AT)2TTCA(GA)2 60 247-279 6 0.76 NA NA NA HM208140
PA01-E10 (GGT)2 T(ACT)2 54 186-210 6 0.43 NA NA NA HM208141
PA02-F8 (AC)4TA(AC)3 TD 166-200 6 0.42 NA NA NA 58
PA01- F10 (CT)10 50 188-240 18 0.48 234-220 2 0.14 58
PN03-A6 (GT)8 60 152-192 20 0.76 160-188 11 0.86 57
PN02-B5B (TCA)4 60 152-234 24 0.79 180-204 4 0.58 57
PN03-C7 (GT)6 50 133-177 20 0.83 159-165 3 0.58 HQ585425
PA02-G11 (CT)11 TD * * * NA NA NA HQ585425
PA02-H1 (AC)3G(CA)3 TD * * * NA NA NA HM208150
PA02-H4 (CA)2CT(CA)5 T(AC)2 TD * * * NA NA NA 58
PA02-H8 (GT)3GCA(TG)3 TD * * * NA NA NA 58
PN03-A5 (TG)8 60 * * * 249-287 4 0.54 57
PN03-F10 (TG)7 60 * * * 165-177 7 0.78 HQ585433
PN02-C6 (TG)8 51.4 * * * 139-145 4 0.29 HQ585422
PN02-F6A (CT)11 60 * * * 154-218 22 0.89 57
PN03-D10 (GT)11 50 * * * 280-300 7 0.66 HQ585426
PN03-F2 (AC)7 60 * * * 241-249 5 0.64 57
PN03-F9 (CA)8 60 * * * 179-275 15 0.88 HQ585432
PN02-A12 (GA)3AT(GAA)3 60 NA NA NA 185-205 3 0.45 57
PN02-B3 (GT)12 50 NA NA NA 139-157 11 0.84 HQ585421
PN02-G10 (CA)8 60 NA NA NA 189-219 7 0.83 57
PN02-G3 (GT)9 60 NA NA NA 294-302 5 0.6 HQ585423
PN02-G7 (AC)7 TD NA NA NA 118 7 0.74 HQ585424
PN02-H7 (AC)8 60 NA NA NA 194-204 4 0.63 57
PN03-D12 (AG)10 50 NA NA NA 220-240 7 0.69 HQ585427
PN03-E7 (GT)9 TD NA NA NA 298 7 0.74 HQ585428
PN03-E9b (AC)7 60 NA NA NA 168-186 6 0.59 57
PN03-F1 (CA)7 50 NA NA NA 184-192 5 0.71 HQ585429
PN03-F3 (GT)8 60 NA NA NA 165-203 8 0.75 HQ585430
PN03-F7 (CA)10 50 NA NA NA 200-212 6 0.76 HQ585431
PN03-G5 (CA)7 60 NA NA NA 126-138 6 0.74 HQ585434
PN03-G8 (CA)7 60 NA NA NA 254-258 3 0.38 57
PN03-H10 (CT)11 60 NA NA NA 222-272 13 0.86 57
PN03-H3 (GA)22 51.4 NA NA NA 198-250 11 0.84 HQ585435
Average     15.58 0.65   7.52 0.64  
  1. * Test of cross-species amplification failed; NA: not analyzed; Ta: primer annealing temperature; TD: touchdown; NM: number of markers; PIC: Polymorphism information content.