Figure 7
Detection of telomere repeat transcripts at the terminal regions of P. ridibundus lampbrush chromosomes. DNA/RNA FISH with (TAACCC)5-biotin on P. ridibundus lampbrush chromosome E (b, b`). (TTAGGG)n-repeat transcripts are visible in small caps at the ends of lampbrush chromosomes (indicated by arrows). Asterisk indicates enlarged region of lampbrush chromosome E. Chromosomes are counterstained with DAPI. Corresponding phase contrast micrographs are shown (a, a`). Arrowheads indicate centromeres. Nu – extrachromosomal nucleoli. Scale bars = 10 μm.
