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Table 2 gDNA concentration measured at 260 nm

From: Isolation of human genomic DNA for genetic analysis from premature neonates: a comparison between newborn dried blood spots, whole blood and umbilical cord tissue

Sample

DBS

DBS average

WB

UC

Storage duration

1 month

3-5 years

6-10 years

11-15 years

15-22 years

3-22 years

(<1 month)

(<1 month)

 

n = 20

n = 25

n = 25

n = 15

n = 30

n = 95

n = 31

n = 31

ng/μl

9.2 ± 1.5

6.2 ± 8.7

11.4 ± 6.9

7.9 ± 2

7.6 ± 4.2

8.3 ± 10.7

40.3 ± 10.9

117.3 ± 112.9

Total DNA (μg)

0.55

0.37

0.68

0.48

0.46

0.49

4.03

70.4

A260:A280

1.7

2.0

2.2

2.5

2.4

2.3

1.8

1.9

  1. p < 0.01 WB vs UC, p < 0.001 DBS (3-22 years average) vs UC total DNA.