Analysis of the tissue expression profiles of 16 porcine β-defensins from 15 tissues using semi-quantitative RT-PCR. GAPDH was used as a control to control for the amount of cDNA and the level of gene expression. (A) The gene expression analysis of β-defensins from a 2-week-old pig. (B) Temporal changes in the gene expression of pBD115 in testes between 2-week-old and 5-month-old pigs. 1, small intestine; 2, tongue; 3, eye; 4, cerebrum; 5, spleen; 6, kidney; 7, liver; 8, lung; 9, stomach; 10, testis; 11, muscle; 12, skin; 13, rectum; 14, trachea; 15, thymus.