Figure 1

DNA damage profiles for GS FLX sequenced amplicons of mtDNA from koala museum specimens. For each koala specimen, the consensus sequence was determined by aligning 454 reads for the individual. Observed differences from the consensus character states were tallied at each nucleotide position for each of the 3 possible non-consensus bases. The total for each possible type of base error relative to the consensus koala sequence was calculated by considering each type of error across all of the nucleotide sites that had the same consensus character state; thus C-T error represents for all sites at which the consensus sequence was cytosine the average proportion of times at which a thymine was present. The percentage for each possible error for each koala is shown. The scale of the y-axis was set to a maximum of 0.7%. The single bar interrupted by hatch marks indicates a value of 2.21% for C-T errors in koala Pci-maex1738.