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Figure 3 | BMC Genetics

Figure 3

From: Detection of nitrogen deficiency QTL in juvenile wild barley introgression linesgrowing in a hydroponic system

Figure 3

Seed germination and plant cultivation. A. Barley seeds weregerminated in the dark at room temperature. B. After 72 h, seedlingsthat had three to five roots of 1.5 to 2.0 cm length and that had not yetstarted to extend the shoot were selected. Each seedling was inserted into a1.5 ml reaction tube (Eppendorf, Hamburg) that was cropped at the bottom.C. The tubes with the seedlings were subsequently plugged into theperforated trays and placed on top of the boxes. D. Throughout theexperiments the roots plunged into the nutrient solution whereas plant shootsgrew on top of the tray.

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