Table 1 Yeast strains used in this study

FY23

MATa leu2Δ1 ura3-52 trp1Δ63

[39]

FY86

MATα leu2Δ1 ura3-52 his3Δ200

[39]

CSY550

MATa leu2Δ1 ura3-52 trp1Δ63 rat8-2

[8]

PDY4

MATa ura3-52 his3Δ200 ssl1-1 rat8-2

[11]

CSY564

MATα leu2Δ1 ura3-52 his3Δ200 rat8-2 [pRAT8.31]

[11]

GY215

MATa ura3-52 trp1Δ63 his4-912δ lys2-128δ bur6-1

G. Prelich

PDY6

MATα leu2Δ1 ura3-52 lys2-128δ rat8-2 bur6-1 [pRAT8.31]

[11]

CSY560

MATa leu2Δ1 ura3-52 trp1Δ63 rat8-2 [pRAT8.31]

[11]

CS41-4.3

MAT? ura3-52 leu2-3 his3-11 trp1-1 ade2-1 bur6:: HIS3 [pbur6-ts/CEN/LEU2]

D. Reinberg

YMH202

MAT? ura3-52 leu2-3 his3-11 trp1-1 ade2-1 bur6:: HIS3 [pBUR6/CEN/URA3]

D. Reinberg

mex67-5

MATa ade2 leu2 ura3 his3 trp1 mex67::HIS3 [pmex67-5/TRP1/CEN]

[29]

yra1-1

MATa ade2 leu2 ura3 his3 trp1 yra1::HIS3 [pyra1-1/TRP1/CEN]

[18]

sub2-85

MATa leu2 ura3 his3 trp1 sub2::kanMX4 [psub2-85/TRP1/CEN]

[40]

yra1-1 bur6-ts

MAT? ade2-1 trp1 ura3 his3 leu2 bur6:: HIS3 yra1::HIS3 [pbur6-ts/CEN/LEU2] [pyra1-1/TRP1/CEN] [pYRA1/URA3/CEN]

This study

sub2-85 bur6-ts

MAT? trp1 ura3 his3 leu2 bur6::HIS3 sub2::kanMX4 [pbur6-ts/CEN/LEU2] [psub2-85/TRP1/CEN] [pSUB2/URA3/CEN]

This study

mex67-5 bur6-ts

MAT? ade2-1 trp1 ura3 his3 leu2 bur6::HIS3 mex67::HIS3 [pbur6-ts/CEN/LEU2] [pmex67-5/TRP1/CEN] [pMEX67 URA3/CEN]

This study

mex67-6 bur6-ts

MAT? ade2-1 trp1 ura3 his3 leu2 bur6::HIS3 mex67::HIS3 [pbur6-ts/CEN/LEU2] [pmex67-6/TRP1/CEN] [pMEX67 URA3/CEN]

This study

mex67-5 ssl1-1

MAT? ade2 trp1? leu2 ura3 ssl1-1 mex67::HIS3 [pmex67-5/TRP1/CEN]

This study

mex67-6 ssl1-1

MAT? ade2 trp1? leu2 ura3 ssl1-1 mex67::HIS3 [pmex67-6/TRP1/CEN]

This study

sub2-85 ssl1-1

MAT? his3 ura3 sub2::kanMX4 [psub2-85/TRP1/CEN]

This study

TOA1 rat8-2

MAT? leu2 his3 ura3 rat8-2

This study

toa1-2 rat8-2

MAT?leu2 trp1 rat8-2 toa1-2

This study

toa1-2 DBP5

MAT? leu2 toa1-2

This study

TOA1 DBP5

MAT? leu2 his3

This study

MY1870

MATα trp1 ura3-52 leu2::PET56 gal2 gcn4D toa1-2

[25]

toa2 (Y68F)

MATα toa2::HIS3 ade1 ura3-52 leu2Δ1 [ptoa2(Y68F)/LEU2/CEN]

[26]

toa2 (F71E)

MATα toa2::HIS3 ade1 ura3-52 leu2Δ1 [ptoa2(F71E)/LEU2/CEN]

[26]

toa2 (W76F)

MATα toa2::HIS3 ade1 ura3-52 leu2Δ1 [ptoa2(W76F)/LEU2/CEN]

[26]

toa2 (W76A)

MATα toa2::HIS3 ade1 ura3-52 leu2Δ1 [ptoa2(W76A)/LEU2/CEN]

[26]

rat8-2 toa2 (Y68F)

MAT? toa2::HIS3 rat8-2 ura3-52 leu2Δ1 trp1Δ63 [ptoa2(Y68F)/LEU2/CEN]

This study

rat8-2 toa2 (F71E)

MAT? toa2::HIS3 rat8-2 ura3-52 leu2Δ1 trp1Δ63 [ptoa2(F71E)/LEU2/CEN]

This study

rat8-2 toa2 (W76F)

MAT? toa2::HIS3 rat8-2 ura3-52 leu2Δ1 trp1Δ63 [ptoa2(W76F)/LEU2/CEN]

This study

rat8-2 toa2 (W76A)

MAT? toa2::HIS3 rat8-2 ura3-52 leu2Δ1 trp1Δ63 [ptoa2(W76A)/LEU2/CEN]

This study