Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

Table 3 Resolution between consecutive steps of the dilution series determined by the P-values of a two-tailed T-test for comparing averages

Assay	Target	Batches	Exp amount DNA (ng/reaction)
			72.0-36.0	36.0-18.0	18.0-9.0	9.0-4.5
I	Tel	7	0.103	0.051	0.013	0.005
	Ref	7	0.029	0.052	0.024	0.022
IV	Tel	4	0.014	0.001	0.000	0.002
	Ref	4	0.010	0.009	0.007	0.012
Assay	Target	Batches	Exp amount DNA (ng/reaction)
			60.0-20.0	20.0-6.7	6.7-2.2
II	Tel	4	0.013	0.007	0.002
	Ref	4	0.019	0.018	0.254
III	Tel	4	0.000	0.001	0.000
	Ref	4	0.032	0.002	0.020

Bold values are non-significant at the 5% level and indicate that there is no difference between the amounts of DNA observed at two consecutive dilution steps.

ISSN: 2730-6844