From: Quantitative criteria for improving performance of buccal DNA for high-throughput genetic analysis
N | All buccal | QC passed | QC failed | P-value t-test or χ2 | p-value Wilcoxon or Exact |
---|---|---|---|---|---|
708 | 628 | 80 | |||
Years since sample collection | 7.9 [5.3, 10.0] | 8.0 [5.6, 10.0] | 5.3 [4.2, 9.0] | <0.0001 | <0.0001 |
Current cigarette smoker (%) | 136 (19.2%) | 116 (18.5%) | 20 (25%) | 0.16 | 0.18 |
Current cigar smoker (%) | 21 (3%) | 17 (2.7%) | 4 (5%) | 0.26 | 0.28 |
Average daily coffee intake (cups) | 1.43 (1.28, 1.59) | 1.39 (1.23, 1.54) | 1.79 (1.16, 2.42) | 0.23 | 0.25 |
Average daily tea intake (cups) | 0.42 (0.33, 0.51) | 0.42 (0.32, 0.51) | 0.42 (0.19, 0.66) | 0.96 | 0.77 |
Average daily sodas | 0.75 (0.62, 0.87) | 0.74 (0.60, 0.87) | 0.85 (0.54, 1.16) | 0.50 | 0.06 |
260/280 Ratio | 1.81 (1.80-1.81) | 1.81 (1.80-1.81) | 1.82(1.80-1.84) | 0.42 | 0.32 |
260/230 Ratio | 1.17 (1.15- 1.20) | 1.18 (1.15-1.21) | 1.14 (1.06-1.21) | 0.38 | 0.39 |
Total DNA (ng/μl)a | 87.4 (82.3-92.8) | 85.6 (80.6-91.8) | 102.5 (87.4-121.5) | 0.06 | 0.05 |
ds DNA (ng/μl)a | 55.7 (52.5-59.1) | 56.8 (53.5-60.3) | 49.4 (41.3-59.1) | 0.12 | 0.09 |
ds/total DNA ratio | 0.66 (0.65-0.68) | 0.68 (0.67-0.69) | 0.52 (0.46-0.57) | <0.0001 | <0.0001 |