Molecular ecology and selection in the drought-related Asr gene polymorphisms in wild and cultivated common bean (Phaseolus vulgaris L.)

Table 5 Primers used for PCR amplifications of candidate genes in P. vulgaris in the Core and wild collections

Gene	Primer Name	F/R	Sequence (5′-3′)	Ta (°C)	Source	Sequence*
Asr1 (region 1)	ADOC01_01_Pv_12	F	GAGGAGACTAAGCCCATAGA	62	IRD	TC2798
Asr1 (region 1)	ASR_TC2798_RV**	R	TGGACAGAAGCCATTCACTCCCAA	62	CIAT
Asr2 (region 2)	ASR_TC2798_FW2	F	AAGCACCACAAGCATCTTGAGCAC	53	CIAT
Asr2 (region 2)	**	R	**	53	**
Asr2	ADOC01_03_Pv_05	F	CCACCACCACAAAGAGGA	60	IRD	CA910244
	ADOC01_03_Pv_06	R	CAAACATTCTTCAAACTTGCTCAGA		IRD

*Accession ID of the original EST sequences used to design the primers. TC accessions are from Gene Index (compbio.dfci.harvard.edu/tgi/).**The reverse primer ASR_TC2798_RV was also used in this case.

ISSN: 2730-6844