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Table 2 Similarity of turbot EST-linked and anonymous sequences against model teleost genomes

From: An Expressed Sequence Tag (EST)-enriched genetic map of turbot (Scophthalmus maximus): a useful framework for comparative genomics across model and farmed teleosts

Model species

Gac

Tni

Ola

Tru

Dre

Total hits E < 10−5 (%)

246 (54.4)

179 (39.4)

186 (41.0)

194 (42.7)

91 (20.5)

 Unique hitsa (%)

210 (48.0)

151 (33.3)

146 (32.9)

162 (35.7)

69 (15.5)

 Multiple hitsb (%)

36 (7.9)

28 (6.3)

38 (8.4)

32 (7.2)

22 (5.0)

EST-linked sequences (E)

 Unique hitsa (%)

121 (60.8)

98 (49.2)

88 (44.2)

93 (46.7)

50 (25.1)

 Unique_L–Lc (%)

98 (49.2)

60 (30.2)

70 (35.2)

66 (33.2)

44 (22.1)

 Multiple hitsb (%)

25 (12.6)

21 (10.6)

32 (16.1)

25 (12.6)

19 (9.6)

Anonymous sequences (A)

 Unique hitsa (%)

89 (36.3)

53 (26.6)

57 (22.4)

69 (27.1)

19 (7.8)

 Unique_L–Lc (%)

78 (31.8)

40 (16.3)

53 (21.6)

66 (26.9)

17 (6.9)

 Multiple hitsb (%)

11 (4.3)

7 (2.9)

7 (2.7)

7 (2.9)

3 (1.2)

Unique hits summary

 Mean size alignment bp

119.0

106.4

110.3

109.0

107.1

 Mean size A-Ed bp

100.0–133.0

91.8–115.0

94.4–119.4

94.7–119.8

84.0–114.0

 Maximum size A-Ed bp

257–504

252–401

215–422

222–438

181–295

 Mean E-value

1.4E-07

4.2E-07

3.6E-07

4.0E-07

6.1E-07

Minimun E-value

1.0E-140

1.0E-116

1.0E-120

1.0E-119

2.0E-62

 Identity % Mean

89.8

89.9

89.0

89.5

88.2

 Identity % Range

79.4–100

80.4–100

79.9–96.7

79.4–100

80.3–96.2

 Retained at E ≤ 10−10 (%)

182 (87%)

122 (81%)

110 (75%)

129 (80%)

50 (72%)

  1. BLASTn matches of 454 turbot microsatellite and SNP flanking sequences (199 EST-linked to 255 anonymous ones) against the stickleback (Gac: Gasterosteous aculeatus), spotted green puperfish (Tni: Tetraodon nigroviridis), medaka (Ola: Oryzias latypes), fugu (Tru: Takifugu rubripes) and zebrafish (Dre: Danio rerio) genomes at E < 10−5 threshold, most of which retained at E ≤ 10−10. aUnique or bMultiple hits: turbot sequences yielding a single significant match or ≥2 significant matches, respectively, against model species genomes. cUnique_L–L: linked markers at the turbot genetic map having unique significant matches with chromosome assignment on the model species genomes. dA-E: size alignment figures in base pairs (bp) for Anonymous (A) to EST-linked (E) markers.